大肠杆菌菌株，携带来自珊瑚色诺卡氏菌（Nocardia corallina）（ATCC 19070）的 NcoI 基因。 一个单位是指在 50 µl 的总反应体系中，37℃ 条件下，1 小时内酶切 1 µg λ DNA 所需的酶量。 对 CpG、dcm 或 dam 甲基化均不敏感。 Why Choose Recombinant Enzymes? What is Star Activity and how can it be avoided? Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?

NotI限制性内切酶能够识别GC^GGCCGC位点，在Tango缓冲体系（+DTT）中于37°C达到最佳切割效果（同裂酶：FauNDI）。 Thermo Scientific传统限制性内切酶产品包含了大量的高品质内切酶，这些酶经过优化，可在五种缓冲液体系中的其中一种中进行酶切，此外，还随酶提供通用Tango缓冲液，以便进行双酶切。 所有内切酶在推荐的缓冲液和反应条件下都具有100%的活性。 为保证内切酶的性能稳定，Thermo Scientific限制性内切酶反应缓冲液还预先混入了BSA，以 …

NOCI can be enabled by specifying CORRELATION = NOCI, and will automatically use all of the states located with SCF metadynamics. Two spin-exchanged versions of a UHF wave function can be requested simply by not turning on metadynamics.

Production of NOCI in aqueous droplets would provide a source of HONO and possibly chlorine atoms in these aerosols, which could then have a role in aerosol chemistry associated with acid

所有 HF 内切酶均符合省时酶（Time-Saver）标准，可在 5-15 分钟内酶切底物 DNA，也可实现过夜酶切而不会造成 DNA 降解。 HF 限制性内切酶在改造时便将性能作为重要指标，可在更宽的条件范围下具有完全活性，最大限度地减少非特异性酶切产物，同时为实验设计 ...

2025年1月31日 · One powerful tool in this exploration is a method called Non-Orthogonal Configuration Interaction (NOCI). While that sounds like a mouthful, it's basically a way to calculate and describe these electronic states more accurately than traditional methods. But like a good plot twist, this method has its own challenges that need to be tackled.

2018年9月11日 · NOCI-MP2 is applied to the binding energy and charge resonance energy in dication and monocation π dimers, as well as didiamantane ethane, and hexaphenylethane. A well-defined set of nonorthogonal determinants are obtained using absolutely localized molecular orbitals (ALMOs), as solutions to the self-consistent field for molecular ...

From these reference states, multiple non-orthogonal states are then created either using SCF metadynamics, or as excitations from the reference determinants, as requested by the $rem variable NOCI_DETGEN. When generating multiple using excitations within an active space, the active orbitals are controlled using the $active_orbitals input ...

2020年5月26日 · 对于从cDNA文库中克隆或者从现成的表达载体再克隆的实验来说，在引物两端加上所选的限制性内切酶的酶切序列和保护碱基（上下游引物分别带有上下游限制性内切酶的酶切序列）。 这样，当PCR完成时，你所得到的目的基因DNA片段两端就带有所选的限制性内切酶的酶切序列。 经过双酶切，目的基因DNA就可以用于载体连接。 还有一些实验需要将目的基因换表达载体，这时就更容易了。 直接使用原来表达载体上的酶切位点进行双酶切，同样可以得到两 …

NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free. NcoI has a …