Flow cytometry and fluorescence microscopy analyses showed that cellular uptake of fluorescence (Dil)-labeled oxLDL was significantly augmented with LPS stimulation. The augmented uptake of Dil-oxLDL was almost completely …

2018年3月8日 · Aside from a TLR4 antagonistic role, oxPAPC binds directly to caspase-4 and caspase-11, competes with LPS binding, and consequently inhibits LPS-induced pyroptosis, IL-1β release and septic...

We found that diverse NLRP3 inflammasome activators rapidly stimulated uniporter-mediated calcium uptake to open mitochondrial permeability transition pores (mPTP) and trigger VDAC oligomerization. This occurred independently of mtDNA or reactive oxygen species, which induce Ox-mtDNA generation.

2023年6月25日 · 血管内皮细胞表面高表达凝集素样 氧化低密度脂蛋白 (LDL)受体-1 (LOX-1)，能够结合并将氧化低密度脂蛋白 (oxLDL)转移到动脉粥样硬化中被巨噬细胞浸润的血管内膜。 巨噬细胞通过几种 清道夫受体 (SR)如SR- A1、CD36和LOX-1感知并结合oxLDL。 在后期内涵体/溶酶体中，溶酶体酸性脂肪酶 (LAL)将LDL颗粒中高度存在的胆固醇酯降解为游离胆固醇和游离脂肪酸。 在内质网 (ER)中， 酰基辅酶A:胆固醇酰基转移酶-1 (ACAT1)促使游离胆固醇形成胆固醇酯。 …

2023年9月1日 · In the present study, polymyxin (PMB) covalently conjugated to PEGylated liposomes (PLPs) were formulated to adsorb LPS through specific interactions between PMB and LPS. In vitro, the experiments demonstrated that PLPs could adsorb LPS, reduce the polarization of macrophages to M1 phenotype and inhibit the formation of foam cells.

2014年10月28日 · Flow cytometry and fluorescence microscopy analyses showed that cellular uptake of fluorescence (Dil)-labeled oxLDL was significantly augmented with LPS stimulation. The augmented uptake of Dil-oxLDL was almost completely …

2019年4月3日 · ox-LDL会通过一些信号通路诱发较强的细胞毒性作用, 最终可导致巨噬细胞、平滑肌细胞、内皮细胞的退化凋亡 。本研究发现相同浓度ox-LDL诱导后, THP-1凋亡比率增加近2倍, 而RAW264.7凋亡比率基本不变。这一结果证明, ox-LDL会引起不同来源的巨噬细胞产生不同的凋亡 ...

Here, we investigated how oxLDL modulates lipopolysaccharide (LPS)-induced inflammatory mediators in RAW 264.7 murine macrophages. Our results showed that oxLDL dramatically induced HO-1 expression, but did not increase pro-inflammatory mediators such as interleukin-1β, tumor necrosis factor-α, iNOS, and monocyte chemoattractant protein (MCP)-1.

2019年4月10日 · Using confluent cultures of Caco-2 cells, a well-known in vitro model of small intestinal enterocytes, we demonstrate a protective role of OX-A against LPS-mediated impairment of the IEB. We propose that OX-A is able to prevent LPS-induced decrease in TEER in this in vitro model by improving the function of tight junctions. In fact, we showed ...

Oxidised lipoprotein (a) [oxLp (a)] is considered as a more potent arteriosclerotic factor than native Lp (a). However, the molecular mechanisms underlying this potency remain unclear. Reactive oxygen species (ROS) possibly act as intracellular second messengers that participate in autophagy stimulation.