This strain may be useful for studying CD8+ T cell activation and differentiation and as a source of donor cells for adoptive transfer for in vivo studies of inflammation, tolerance and anti-viral responses.

The most commonly used TCR Tg mouse strain specific to LCMV is the P14 TCR Tg mouse. This mouse expresses TCR specific to the MHC-I-specific LCMV glycoprotein (GP) 33–41 peptide [ 20 ]. With an acute strain of LCMV, such as the Armstrong strain, P14 TCR Tg T cells were used to study effector function and memory formation of anti-viral CD8 + T ...

2023年2月1日 · By generating P14 TRex mice with the same specificity as the widely used LCMV-P14 TCR transgenic mouse, we demonstrate increased avidity of Trac -targeted TCRs over transgenic TCRs, while...

The Rag2 Knockout/Transgenic LCMV-P14 T Cell Receptor line is homozygous for a transgene that encodes a T cell receptor that is specific for a peptide (P14) from the lymphocytic choriomeningitis virus (LCMV) presented by the MHC class I molecule H2-Db.

We examined T cell development and T cell repertoire in transgenic mice expressing a single T cell receptor (TCR) alpha chain derived from the H-2Db-lymphocytic choriomeningitis virus (LCMV)-specific cytolytic T lymphocyte (CTL) clone P14. To generate these alpha P14 mice, mice transgenic for the P1 …

By generating P14 TRex mice with the same specificity as the widely used LCMV-P14 TCR transgenic mouse, we demonstrate increased avidity of -targeted TCRs over transgenic TCRs, while preserving physiologic T cell development.

2024年5月28日 · We transferred Thy1.1 naïve P14 TCR transgenic CD8 T cells, which specifically react with the LCMV GP 33–41 peptide presented by H-2D b, into Thy1.2 WT host mice and infected them with vaccinia...

(A) P14 mice expressing a transgenic Va2 + and Vb8 + TCR were crossed to twp mice. Single-positive CD8 + thymocytes expressing the transgenic TCR were quantified by flow cytometry.

Bioz Stars score, Techniques, Protocol Conditions and more for P14 Mice, supplied by The Jackson Laboratory. Data for P14 Mice gathered from related PubMed articles.

2020年9月8日 · P14 cells were adoptively transferred into mice 1 day prior high or low-dose LCMV clone 13 infection. Animals were sacrificed after 14 days. CD8 + P14 cells were stimulated with anti-CD3 and...

2024年6月28日 · The Cx3cr1 CreER mouse strain (JAX stock #020940) and the R26-eYFP mouse were crossed to P14 mice to obtain an inducible reporter for CX3CR1 expression P14 cells. After 30 days, we treated mice with tamoxifen to induce YFP expression selectively in the transferred LLEC P14 cells.

2025年2月25日 · (A) Representative coronal sections of Slc17a6-Cre, Ago2 flox/flox mice and littermate controls at P14 visualized with Nissl staining (n = 6 biological replicates for each genotype). Scale bar, 500 μm. (B and C) The high magnification in the regions boxed in (A) is to show reduced cortical thicknesses in Slc17a6-Cre, Ago2 flox/flox mice at P8 ...

P14 mice expressing a transgenic TCR specific for the lymphocytic choriomeningitis virus glycoprotein p33 epitope were used to study the induction of CTL effector activity by a variety of ligands. Surprisingly, p33 variants which are weaker agonists for the P14 TCR than the wild-type p33 peptide wer …

By generating P14 TRex mice with the same specificity as the widely used LCMV-P14 TCR transgenic mouse, we demonstrate increased avidity of Trac-targeted TCRs over transgenic TCRs, while preserving physiologic T cell development. Together, our results support that the TRex methodology is an advanced tool to study physiological antigen-specific ...

2020年12月18日 · T cell receptor (TCR) Tg mice are extremely useful in studying T cell-mediated immune responses because the majority of T cells in these mice express specific TCRs for partner antigens. In this review, we discuss the important studies utilizing TCR Tg mice to unveil underlying mechanisms of T cell-mediated immunopathogenesis during viral ...

2022年5月27日 · By performing lineage tracing using reporter mice in which mCherry marked Rgs16-expressing cells, we identified that Rgs16 + CD8 + tumor-infiltrating lymphocytes (TILs) were terminally differentiated, expressed low levels of T cell factor 1 (Tcf1), and underwent apoptosis as early as 6 days after the onset of Rgs16 expression.

2025年3月14日 · a Heart weight to tibial length (HW/TL) ratio in control and CKO mice at p1 and p14, respectively (n = 9 mice for p1 and 10 mice for p14). b Representative whole images of control and CKO ...

Human p14 ARF (p19 ARF in mouse) is a pro-apoptotic cell cycle regulator frequently inactive in human tumors (1). Basal expression of p14 ARF is low in most cell types, but accumulation of this protein occurs in response to oncogene expression (2,3).

2020年8月25日 · We have characterized the landscape of exhausted P14 cells isolated from six tissues (blood, spleen, BM, LNs, lungs, and liver) harvested from mice chronically infected with LCMV clone 13 and classified them into five main functional phenotypes: T M, T P, T E, T I, …