Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines

Thermo Scientific Pierce SMCC 是一种异双功能交联剂，包含N-羟基琥珀酰亚胺(NHS)酯和马来酰亚胺基团，可实现胺基和巯基分子的共价结合。 适用于蛋白质交联和巯基交联应用。

Thermo Scientific Pierce Sulfo-SMCC No-Weigh 规格是一种水溶性胺对巯基交联剂，含有 NHS 酯和马来酰亚胺反应性基团，位于中等长度环己烷间隔臂（8.3 埃）的相对两端。 Sulfo-SMCC No-Weigh 规格的特点： •反应性基团：sulfo-NHS 酯和马来酰亚胺

Sulfo-SMCC crosslinking reagent has an 8.3 angstrom non-cleavable spacer arm and its maleimide group reacts with sulfhydryls (thiols), while the Sulfo-NHS ester is reactive toward primary amines. Sulfo-SMCC can be used to pro-duce antibody-protein crosslinked conjugates or efficiently label cell surface proteins.

The Thermo Scientific™ SMCC and its water-soluble analog Sulfo-SMCC are heterobifunctional crosslinkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.

Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC) is a non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds.

Thermo Scientific Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm (16.2 angstroms). Features of LC-SMCC

NHS esters react with primary amines at pH 7~9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5~7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH.

单克隆的SMCC抗体衍生化与低(聚)核苷酸结合，用于两步放射免疫疗法中；双异官能团交联试剂，将胺以及巯基反应性合并入扩展间隔片，典型地，在pH7.5缓冲液(6.5-8.5)中，通过酰胺键偶联至包含伯胺的分子，在pH6.8缓冲液(6.5-7.0)中，通过硫醚连接，次级偶联至特别 ...

SMCC is a non-cleavable and membrane permeable crosslinker with a spacer arm of 8.3angstrom. SMCC [4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester] *CAS 64987-85-5* is manufactured by AAT Bioquest