
Length-dependent Binding of Human XLF to DNA and
2007年4月13日 · Similar to the soluble XLF-Myc-His, GST-XLF increased the dimer ligation 7- and 11-fold at 3- and 10-fold ratios of GST-XLF to XRCC4·DNA ligase IV (Fig. 2D, lanes 1 versus 2 and 3). Bead-bound XLF-Myc-His was also tested in the ligation.
XLF/Cernunnos: An important but puzzling participant in the ...
XLF (XRCC4-like factor), also called Cernunnos, was originally identified as the protein defective in a subset of RS-SCID patients who, in addition to immunodeficiency and radiosensitivity, displayed growth retardation, microcephaly, and lymphopenia.
HTRF Anti-GST mAb XL665-Conjugate, 1,000 Assay Points
MAb Anti GST-XL665 is an IgG2a raised against Glutathione S-transferase labeled with XL665. It has been shown to react with GST-tagged fusion proteins from a large number of expressing vectors.
XLF Interacts with the XRCC4-DNA Ligase IV Complex to Promote …
The identification of XLF by a yeast two-hybrid screen for XRCC4 interactors suggested that XLF might functionally cooperate with the XRCC4-DNA Ligase IV complex. To begin to explore this possibility, we generated a glutathione S-transferase (GST)-XLF fusion protein in bacteria, purified this, and then used it in protein interaction studies.
XRCC4 deficiency in human subjects causes a marked neurological ...
2015年10月1日 · For expression and purification of Glutathione S-transferase (GST) tagged XLF protein, Escherichia coli BL21 (DE3) cells were transformed with pGEX6-GST-XLF plasmid. The GST-XLF protein was induced with isopropyl-β-D-1-thiogalactopyranoside. The cells were harvested and lysed, and cleared lysate was loaded onto a GSTrap HP column (GE ...
DNA-PK and ATM phosphorylation sites in XLF/Cernunnos are …
2008年10月1日 · GST-XLF 240–299 was immobilized on GST-beads and phosphorylated by DNA-PK as above, except that reactions contained 800 μg of GST-XLF 240–299 and 170 μg of purified DNA-PK complex. Phosphorylation reactions contained 0.25 mM ATP, while mock phosphorylation contained DNA-PKcs, Ku, DNA but no ATP.
Akt-Mediated Phosphorylation of XLF Impairs Non-Homologous …
Here, we report that Akt phosphorylates XLF at Thr181 to trigger its dissociation from the DNA ligase IV/XRCC4 complex, and promotes its interaction with 14-3-3β leading to XLF cytoplasmic retention, where cytosolic XLF is subsequently degraded by SCF β …
XLF-Cernunnos promotes DNA ligase IV–XRCC4 re-adenylation …
2008年12月4日 · We propose a model in which XLF, by in situ recharging DNA ligase IV after the first ligation event, promotes double stranded ligation by a single LX complex. DNA non-homologous end-joining (NHEJ) is the major mechanism for the repair of radiation induced DNA double strand breaks (DSBs) in mammalian cells.
XLF Antibody #2854 - Cell Signaling Technology (CST)
Polyclonal Antibody for studying XLF. Validated for IP, WB. Available in 2 sizes. Highly specific and rigorously validated in-house, XLF Antibody (CST #2854) is ready to ship.
Loss of DNA ligase IV prevents recognition of DNA by double …
To directly test the hypothesis that formation of an XRCC4/XLF complex inhibits DNA binding by XRCC4 and XLF, we coexpressed C-terminally his-tagged XRCC4 (XRCC4-his 6) and N-terminally GST-tagged XLF (GST-XLF) in E. coli, used Ni-NTA and glutathione sepharose affinity chromatography in tandem to isolate complexes that contain XRCC4-his 6 and ...
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