An E. coli strain that carries the BglII gene from Bacillus globigii (ATCC 49760). This product is related to the following categories: Restriction Endonucleases B Products,

Thermo Scientific BglII restriction enzyme recognizes A^GATCT sites and cuts best at 37C in buffer O. Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one o

BglII has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10237497. Learn more. We are excited to announce that all reaction buffers are now BSA-free.

从 2021 年 4 月开始，NEB 将含有 BSA 的反应缓冲液转换为含有 重组白蛋白（rAlbumin） 的缓冲液，用于限制性内切酶和某些 DNA 修饰酶。 如需了解更多信息，请访问 www.neb.cn/BSA-free。 大肠杆菌菌株，携带有克隆自球芽孢杆菌（Bacillus globigii）（ATCC 49760）的 BglII 基因。 一个单位是指在 50 µl 的总反应体系中，37℃下，1 小时内酶切 1 µg λ DNA 所需的 BglII 酶量。 …

Bacillus subtilis (Ehrenburg) Cohn ATCC 49760, deposited as Bacillus globigii, is the source strain for the restriction enzymes BglI and BglII. Its complete sequence and full methylome were determined using single-molecule real-time (SMRT) sequencing.

Bgl II is a 223-amino acid restriction endonuclease, originally isolated from Bacillus globigii 16, that recognizes and cleaves the DNA sequence AGATCT. Recently, we reported the 1.5 Å...

This paper describes the characterization of an intracellular β-glucosidase enzyme BGLII (Cel1a) and its gene (bgl2) from the cellulolytic fungus Trichoderma reesei (Hypocrea jecorina). The expression pattern of bgl2 is similar to that of other ...

We show that both enzymes share a similar α/β core, but in BglII, the core is augmented by a β-sandwich domain that encircles the DNA to provide extra specificity.

1999年9月23日 · We compare the structure of BglII to endonuclease BamHI, which recognizes a closely related DNA site (GGATCC). We show that both enzymes share a similar alpha/beta core, but in BglII, the core is augmented by a beta-sandwich domain that encircles the DNA to provide extra specificity.

BglII is a type II restriction endonuclease of 223 amino acids that targets the sequence AGATCT by encircling DNA from the major groove side (Lukacs et al. 2001). It is composed of two identical monomers that are bound together by two pairs of a-helices .