Plasmid pCas9 from Dr. Luciano Marraffini's lab contains the insert tracr/Cas9 and is published in Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508. This plasmid is available through Addgene.

Plasmid pCas9-CR4 from Dr. Kristala Prather's lab contains the insert Cas9 nuclease and is published in Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096. This plasmid is available …

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. The maps, notes, and annotations in the zip file on this page …

In pCas9, we 1) replaced the promoter of gRNA-pMB1 with PrhaB, 2) changed the replicon of pCas into a non-temperature-sensitive replicon, and 3) added the sacB gene for …

2021年4月15日 · The clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (Cas9)-based genome editing tool pCas/pTargetF system that we …

By using the enhanced green fluorescent protein reporter plasmid pGNtsf1, we demonstrate that pCas9-U6sgRNA driven by endogenous U6 promoter (pCas9-mU6sgRNA) mediated very high …

Plasmid pCas9 from Dr. Howard Hang's lab contains the inserts chloramphenicol acetyl transferase and pUC19 origin of replication and is published in Appl Environ Microbiol. 2021 …

Bacterial plasmid with a temperature-sensitive replicon encoding Cas9, an inducible single guide RNA (sgRNA), and the λ Red recombination system. The lac repressor binds to the lac …

2015年10月14日 · Cas9 is an endonuclease that targets a specific DNA sequence that can be easily programmed by a plasmid encoded target sequence of 20 bp 18. The only requirement …

2021年3月25日 · The clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (Cas9)-based genome editing tool pCas/pTargetF system that we …