2011年1月16日 · We describe an approach that enables live-cell imaging of single endogenous labeled mRNA molecules transcribed in primary mammalian cells and tissue. We generated a knock-in mouse line with an...

2014年1月24日 · The MCP×MBS mouse provides a distinctive tool for monitoring the dynamics of single endogenous mRNA in live mammalian cells and tissues. Our results with the β-actin gene suggest that the technique could be generally applicable to other genes to investigate the effect of the tissue microenvironment on single-cell gene expression.

2019年12月1日 · Microbubbles (MBs) are known to serve as an amplifier of the mechanical effects of ultrasound, which combined with ultrasound are widely used in brain. The goal of this study is to investigate the effect of oscillating MBs on the neuronal activity in the central nervous system (CNS) of mammals.

2016年1月1日 · By imaging hippocampal neurons or brain slices from MCP × MBS mice, the dynamics of mRNA, such as transcription, transport, and localization, can be studied at single mRNA resolution. In this chapter, we explain the basics of MCP × MBS mice and describe methods for utilizing these animals.

2019年4月1日 · The MCP mouse is a transgenic mouse that expresses MCP-GFP in every cell type. The Actb-MBS and MCP mice were crossed and bred to double homozygosity to generate the MBS×MCP mouse line (Park et al., 2014). In the MBS×MCP mouse, all endogenous β-actin mRNAs are labeled with up to 48 MCP-GFPs because each MBS binds a dimer of MCP-GFP.

By imaging hippocampal neurons or brain slices from MCP×MBS mice, the dynamics of mRNA, such as transcription, transport, and localization, can be studied at single mRNA resolution. In this chapter, we explain the basics of MCP×MBS mice and describe methods for …

2023年9月6日 · To demonstrate that the observed analgesic effects of GNE-3565 are strictly due to Nav1.7 inhibition, we constructed a mouse line (referred to as the Nav1.7 modified binding site [MBS] mouse) with the mutation at the aryl-sulfonamide-binding site (YWV → SRL) that decreases the affinity of GNE-3565 by >100-fold .

To overcome this limitation, we present a genetically encoded RNA indicator (GERI) mouse for intravital chronic imaging of endogenous Arc messenger RNA (mRNA)—a popular marker for memory trace ...

6 天之前 · To confirm targeting specificity, ncMBs were incubated with CD11b-expressing mouse bone marrow-derived macrophages (BMDMs) and non-CD11b-expressing B16F10 and D4M.3A melanoma cells. ... SpeDex MBs were then added to the solution of aCD11b-SH at a ratio of 0.2 equivalents of antibody per maleimide and rotated end-over-end for 16 h at 4 °C to ...