2024年9月19日 · Contrarily, the promotion of more evenly distributed CD4 + and CD8 + T cell proliferation was aided by the presentation of a single activation signal (pHLA scaffold and CD80 scaffold) (Figures 2 E and 2F).

2023年6月25日 · 然而，疫苗的效力取决于抗原提呈细胞 (APC)的功能，包括运输、抗原处理、共刺激与共抑制以及对肿瘤免疫抑制的敏感性。 例如，DC是一种APC，通过递送pHLA和强大的共刺激配体如CD80、CD86和CD137L，在启动和维持抗肿瘤CD8 T细胞应答中发挥关键作用。

2024年7月18日 · Resuspend cells in FACS buffer. Optionally, before surface staining, add 2-4 nM APC-conjugated peptide-HLA (pHLA) multimer (s) of interest to the effector-only and/or CFSE proliferation samples for 15 min at 4°C to assess antigen-specific TCR surface expression and frequency of antigen-specific effector cells.

The TCRs expressed by CD8 killer cells recognize pHLA complexes on tumor cells and are necessary and sufficient to mediate durable remissions across various types of cancer immunotherapies, including checkpoint inhibitors (CPIs), adoptive cell therapies and cancer vaccines (10 – 12).

Similar experiments with mutated cognate pHLA-A2 resulted in almost linear dependence (Fig. 4 g ), suggesting that the effect of enhancement is mostly determined by CD8–pMHC-I interactions.

2023年2月1日 · In this study, we investigated the mechanism of the high-affinity CD8 + T cell response to immunodominant HIV-1 epitope Gag-TL9 by reporting its TCR-pHLA ternary-complex structure.

2022年8月10日 · Finally, we took advantage of a collection of T cells expressing affinity-optimized NY-ESO-1-specific TCRs to interrogate the individual and combined impact of peptide:HLA and TCR-pHLA affinities on overall CD8 T cell responses.

The mutation in cognate pHLA-A2 significantly decreased the binding of QDGL9- HLA-A2 conjugate to live CTL as well. These experiments clearly show a significant contribution of CD8-MHC-I ...

CD8 molecules are known to significantly increase the TCR-based recognition of peptides presented by HLA class I molecules. 20 This CD8 dependency, however, can be overcome by certain higher affinity TCRs if their binding to the pHLA complex is greater than the binding affinity of HLA class I to CD8. 21 22 With such TCRs, desired functions of ...

2007年4月1日 · Human CD8 is a T cell coreceptor, which binds to pHLA I and plays a pivotal role in the activation of cytotoxic T lymphocytes. Soluble recombinant CD8 alphaalpha has been shown to antagonize T cell activation, both in vitro and in vivo. However, because of a very low affinity for pHLA I, high concen …