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ExM的核心是 通过让组织样本均匀膨胀突破传统显微镜的衍射极限，从而实现超分辨率成像。 传统显微镜的分辨率大约在200纳米左右，而ExM可以通过膨胀样本，提供超分辨率的成像效果，达到接近纳米尺度的分辨率。 膨胀后的样本体积通常是原来的100倍，像被充气的物体一样，组织几乎呈现出半透明状。 ExM的一个重要优势是， 它能够使用普通的荧光显微镜就实现纳米级别的成像分辨率，这使得这项技术大大降低了高端超分辨率显微技术的门槛。 因此，ExM技术广泛 …

2024年10月11日 · Here, we present an ExM protocol that achieves ~20× expansion (yielding <20-nm resolution on a conventional microscope) in a single expansion step, achieving the performance of iterative...

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2022年2月18日 · Ten-fold Robust Expansion Microscopy (TREx) is a new method for 10-fold physical expansion of specimens in one round of gel embedding, enabling nanoscale imaging of antibody stains and ultrastructural detail.

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We report here an ExSTED imaging protocol that allows for below 10 nm two-dimensional (2D) resolution and isotropic 50–70 nm three-dimensional (3D) resolution in fixed cells. We first aimed to optimize resolution and contrast in ExM toward the routine imaging of …

exm是excuse me的缩写。 因为在网上，大家聊天回复都讲求速度，也可以说是因为大家都很懒。 所以就把excuse me缩写成为exm。 excuse me网络含义：就是“你，他妈在逗我”、“什么鬼?!”、“卧槽这都可以?”有疑问和嘲讽的含义。 excuse me基本意思：请问，劳驾 ...

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2022年2月25日 · 膨胀显微成像技术（expansion microscopy，ExM）是一种新型超分辨成像技术。 该技术借助可膨胀水凝胶均匀地物理放大生物样本，在常规光学成像条件下实现超分辨成像。