A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum.

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。 该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但 …

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B …

Roswell Park Memorial Institute medium is commonly known as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. It is used for the growth of human lymphoid cells.

Roswell Park Memorial Institute（RPMI）1640 培养基相比其他培养基之所以具有独特的效果，是因为其中含有还原剂谷胱甘肽和高浓度的维生素，包括伊格尔最低必需培养基（EMEM）或Dulbecco 改良的 Eagle 培养基（DMEM）所不具备的某些维生素。

RPMI 1640 is also used for suspension cultures or monolayer human leukemic cells. This formulation is produced with L-glutamine. Complete Certificate of Analysis available for each production lot, along with full formulation information. Manufactured in a facility that complies with ISO 13485 certified quality system.

最初设计为需要补充血清，但在无血清条件下，RPMI1640也表现出可支持多种细胞系的生长。 它还广泛用于细胞融合和杂交细胞的生长。 RPMI 1640培养基 上下游产品信息 上游原料

RPMI-1640是改进型的McCoy 's 5A培养基，使用碳酸氢盐缓冲系统。 RPMI-1640完全培养基（含10%血清，1%双抗）含有足量血清及双抗，可以直接用于细胞培养，如无特别需要不用额外再添加血清和双抗。

Shop MilliporeSigma SIGMA RPMI-1640 Medium 10 x, Without L-glutamine, folic acid and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

RPMI 是Moore 等人针对人体正常细胞及神经细胞体外培养开发的一系列培养基。 其中以RPMI 1640最为常用。 作为McCoy's 5A培养基的改良版, 这款培养基被专门用以淋巴母细胞的悬浮培养，目前则被广泛用于多种种属来源的贴壁细胞株的培养。 该培养基可辅以5-20%的胎牛血清，也可支持无血清状态下的细胞生长。 建议用户查阅相关文献，以获取针对不同细胞系的生理生长要求来对培养基进行相关组分的补充。 【成分表】 【使用方法】 从2-8°C的储存条件中取出，读取 …