Detection of singlet oxygen production by ESR - Nature
1979年5月1日 · Lion et al.10 have proposed a new method to detect 1O2 involving the generation of stable nitroxide radicals when 1O2 reacts with the sterically hindered amine 2,2,6,6,-tetramethylpiperidin.
EPR Spin-Trapping for Monitoring Temporal Dynamics of Singlet …
2024年4月28日 · Here, we combine spin-trapping electron paramagnetic resonance (EPR) and time-resolved fluorescence spectroscopies to track in real time the involvement of 1 O 2 * during photoprotection in plant thylakoid membranes.
ESR测量单线态氧猝灭活性的单线态氧检测系统的 ... - X-MOL
单线态氧（1o2）具有很高的氧化性，并具有很强的细胞毒性作用。我们试图建立单线态氧生成系统和esr检测方法的最佳组合，以测量各种物质的淬灭活性。
通过 ESR 检测单线态氧的产生,Nature - X-MOL - X-MOL科学 ...
10 提出了一种检测 1o2 的新方法，包括当 1o2 与位阻胺 2,2,6,6,-四甲基哌啶反应时产生稳定的氮氧自由基。 使用这种方法检测中性水溶液中的 1O2 时，我们没有发现自由基的产生。
Electron spin resonance spectroscopy for the study of …
2014年3月1日 · Electron spin resonance (ESR) spectroscopy is a direct and reliable method to identify and quantify free radicals in both chemical and biological environments. In this review, we discuss the use of ESR spectroscopy to study ROS generation mediated by nanomaterials, which have various applications in biological, chemical, and materials science.
EPR/ESR测试空位、自由基原理及制样步骤 - 知乎
自旋捕获技术 是将一不饱和的抗磁功能基团（自旋捕获剂，一般为氮酮类和亚硝基化合物）加入反应体系，产生EPR可以检测的自由基的技术。 如：以5，5-二甲基-1-吡咯啉-N-氧化物（5，5-Dietyl-1-Pyrroline -N-oxide， DMPO）为自旋捕获剂（实际检测体系DMPO控制在2-5 mM），结合ERP波谱技术，可以用来检测体系产生的 OH、超氧阴离子自由基等。 DMPO捕获不同类型自由基的模拟谱： 常见的自旋捕获剂如下： 2、低温、光照、不同气氛条件下的原位EPR实验. 许多 …
ESR study of singlet oxygen generation and its behavior during …
2015年3月16日 · In this study, 1 O 2 produced from the photo-oxidation of P3HT was detected using ESR with 2,2,6,6-tetramethylpiperidine (TEMP) acting as the spin trap. It is important to confirm the generation of 1 O 2 with a method
ESR detection of 1O2 reveals enhanced redox activity in ... - PubMed
The detection of 1O2 was performed using a trapping probe, 2,2,6,6-tetramethyl-4-piperidone, coupled with electron paramagnetic resonance spectroscopy. In addition, we have shown that, together with O2 generation, LEVL illumination increases the reductive capacity of the cells, which explains the difficulties encountered in 1O2 detection.
Detection of singlet oxygen by EPR: The instability of the nitroxyl ...
2022年2月20日 · ESR evidence of the photogeneration of free radicals (GDHB−, O2−) and singlet oxygen (1O2) by 15-deacetyl-13-glycine-substituted hypocrellin B
单线态氧的检测及分析应用研究进展 - 百度文库
【摘 要】综述了单线态氧(1o2)的测定方法.介绍了单线态氧的esr、磷光光度、分光光度、化学发光和荧光法的基本原理、方法研究进展和分析应用情况,并重点介绍了化学发光法和荧光法,同时比较了这几种方法的特点.