22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line expresses prostate specific antigen (PSA).

22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line expresses prostate specific antigen (PSA).

In nude mice, the line forms tumors with morphology similar to that of the xenografts, and like the parental CWR22 and CWR22R xenografts, this cell line expresses prostate specific antigen. Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis.

In nude mice, the line forms tumors with morphology similar to that of the xenografts, and like the parental CWR22 and CWR22R xenografts, this cell line expresses prostate specific antigen. Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis.

胞+100mL完全培养液)。若 需单独购买小包装的22RV1 细胞完全培养液(100mL/200mL),可通过本库微信公众号(CELLCELL 或扫描下方�. 养瓶进行细胞传代。请不要继续使用原培养瓶的培养液,本 �. 培养液不含抗生素。用户可根据自己实验室具体情况选择加双抗(100U/mL 青�.

前列腺特异抗原PSA。 二羟基睾丸脂酮可轻微刺激细胞生长,经western blot 检测溶解产物与雄激素�. 体抗体起免疫反应。EGF 可刺激细胞生长,但 TGF-β1. 不能抑制细胞生长。近期证实22RV1 可生产高滴度. 的人逆转录病毒XMRV�. 在裸鼠体�. (i. 您选择我们的服务! 中国科学院干细.

22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. Service for Guidelines, their own country. The cell line expresses prostate specific antigen (PSA).

22RV1细胞贴壁和生长都较慢，传代或消化处理后，48h内不要移动，以免影响细胞贴壁。 前列腺癌细胞，该细胞能产生逆转录病毒需要在生物安全等级2级的实验室环境中操作细胞。 22RV1细胞表达前列腺特异抗原PSA。 二羟基睾丸脂酮可轻微刺激细胞生长，经western blot检测溶解产物与雄激素受体抗体起免疫反应。 EGF可刺激细胞生长，但TGβ-1不能抑制细胞生长。 近期证实22RV1可生产高滴度的人逆转录病毒XMRV。 在裸鼠体内可成瘤。 Yes, forms tumors in nude …

在超净台内把培养瓶里的培养液倒至废液缸, 用1× PBS(T25 cm2 培养瓶添加约2~3mL,T75 cm2 培养瓶约4~5 mL) 洗涤细胞1~2 次,以去除残余的培养液和血清( 血清含有胰酶的抑制因子); 加入2 倍胰酶体积的完全培养液终止消化, 并轻吹打细胞数次, 使所有细胞彻底脱壁;( 注意:吹散细胞时注意要轻柔, 尽量不产生气泡或尽可能产生少量气泡。 ); 用10 mL 移液管转移细胞悬液到一支50 mL 离心管中, 同一批次的细胞可以合并收集在一起,视情况用适量PBS 将培养瓶里的残余细胞洗下来, …

2023年11月14日 · 22RV1可表达前列腺特异抗原，对雄激素受体抗体呈阳性反应。 表皮生长因子 （EGF）可促进细胞生长，二羟基睾丸脂酮促进作用轻微，而 转化生长因子β-1 （TGF β-1）不能抑制细胞生长。 最近的研究还表明，22RV1细胞可产生大量的 人类逆转录病毒 异种小鼠白血病病毒相关病毒（xMRV）。 该细胞常用于癌症相关研究，如靶向治疗、耐药机制和毒理学，也常用于建立前列腺肿瘤异种移植模型。 22RV1为上皮细胞样，贴壁生长，在裸鼠体内可成瘤。 有研 …

培养条件：胎牛血清至最终浓度为10％。 环境：空气，95％;二氧化碳（CO2），5％；温度，37℃. 细胞描述：细胞系表达前列腺特异性抗原（PSA）。 通过Western印迹分析，生长受到二羟睾酮的弱刺激，并且裂解物与雄激素受体抗体发生免疫反应。 生长受表皮生长因子（EGF）刺激，但不被转化生长因子β-1（TGFβ-1）抑制。 最近，已显示22Rv1前列腺癌细胞产生高效价的人类逆转录病毒XMRV（异嗜性鼠白血病病毒相关病毒）。

2020年9月2日 · LNCaP, PC3, and 22Rv1 were commonly used cell lines in the research of prostate cancer. The biological characteristics of these three common prostate cancer cell lines are introduced herein. The LNCap cell line was established from lymph node metastasis specimens of prostate cancer patients by Horoszewicz et al [4].

2012年7月27日 · 22Rv1 is a common prostate cancer cell line used in xenograft mouse experiments as well as in vitro cell culture assays to study aspects of prostate cancer tumorigenesis. Recently, this cell line was shown to harbor multiple copies of a gammaretrovirus, called XMRV, integrated in its genome.

Results: We found that 22Rv1 carries mixed genetic ancestry. The main ancestry proportions for this cell line were 0.41 West African (AFR) and 0.42 European (EUR). In addition, we verified the previously reported racial identifications for PC3 (0.73 EUR), DU145 (0.63 EUR), MDA-PCa-2b (0.73 AFR), and RC-77T/E (0.74 AFR) cell lines.

We have treated four prostate tumor cell lines, DU-145, PC-3, LNCaP and 22RV1 with various concentrations of cisplatin in order to check for influence on viability and for onset of apoptosis induction.

To the best of our knowledge, the first studies associating EVs with drug-resistance in prostate cancer were performed by our research group. These studies centred around docetaxel and included analysis of 22Rv1 and DU145 cell lines and their respective docetaxel-resistant variants 22Rv1RD and DU145RD.

2022年3月1日 · Modest, up to 1.7-fold increase in SIRT3 expression was also observed in 22RVI and DU145 cells by BETi treatment. In contrast, all BETi decreased the expression of SIRT3 in PC3 cells, with up to 60% decrease (P < 0.05) by JQ1.