2023年1月23日 · We carried out live cell imaging of 293T cells co-transfected with LRP6-EGFP and pArl13b-mKate2 to examine the kinetics of LRP6 localizing to cilia upon serum starvation.

The chimera WNT3aFZD4 efficiently induced β-catenin-mediated luciferase activity. This activity was increased 40-fold compared with basal when LRP6 was stably cotransfected, proving that the chimera WNT3aFZD4 can also interact efficiently with LRP6.

2003年1月2日 · Here we show that Kremen2 (Krm2) regulates Dkk2 activity during Wnt signaling. In human 293 fibroblasts transfected dkk2 activates LRP6 signaling. However, co-transfection of krm2 blocks the ability of Dkk2 to activate LRP6 and enhances inhibition of Wnt/Frizzled signaling.

2013年9月26日 · RAB8B depletion reduces LRP6 activity, β-catenin accumulation, and induction of Wnt target genes, whereas RAB8B overexpression promotes LRP6 activity and internalization and rescues inhibition of caveolar endocytosis. In Xenopus laevis and Danio rerio, RAB8B morphants show lower Wnt activity during embryonic development.

2004年4月5日 · We have investigated this issue through a structure–function analysis of Frizzled and LRP proteins that have been implicated in Wnt1 signaling. Consistent with other reports, we find that...

293T-LRP6-beta-propeller-3-4-MESD-Middle cell line was generated using a lentiviral vector expressing the human LRP6 sequence. Figure 1: Characterization of human LRP6 overexpression in the 293T-LRP6-beta-propeller-3-4-MESD stable clone using FACS.

2001年5月1日 · We found that alkaline phosphatase (AP)-tagged Dkk1 (Dkk1–AP) binds effectively to 293T cells transfected with LRP6 (Fig. 1e). This interaction is specific, as Dkk1–AP does not bind to fz8...

2007年9月9日 · Here we show that Rspo1 regulates Wnt signaling by modulating levels of LRP6 on the cell surface, through inhibition of DKK1-dependent internalization of LRP6. To further investigate the role of RSpo1 in the activation of the Wnt pathway, we generated a HEK-293 stable cell line expressing a TCF luciferase reporter plasmid.

2007年8月13日 · In the present study, using a new, highly sensitive anti-LRP6 monoclonal antibody (MAb), we demonstrate that endogenous LRP6 is posttranslationally modified by glycosylation, is present at the membrane in a dimeric form, and is phosphorylated in response to Wnt in a sustained but ligand-dependent manner.

To test whether LRP6-LDLRR mutants underwent ligand-mediated endocytosis, 293T cells expressing either LRP6-WT or a representative minimally Wnt active FH mutant, S1317P, were treated with Wnt3a for various periods and cell surface levels of LRP6 analyzed by flow cytometry.