Human embryonic kidney fibroblast 293T cells lacking expression of intrinsic MLH1 because of MLH1 promoter methylation were cotransfected with a pSG5 expression vector containing a full length ...

2024年8月1日 · HEK-293T cells were transfected with MLH1-HA in the absence or presence of PMS2-Flag. Cells were then collected at indicated time points after CHX treatment for degradation analysis.

PMS2 Knockout HEK293T Cells (PMS2基因敲除HEK293T细胞)是通过同时表达Cas9、目的基因sgRNA和puromycin抗性基因，并实现了目的基因CRISPR敲除的HEK293T细胞。 本细胞中目的基因的敲除已经通过T7EI法的验证。

As a control, unlabeled MLH1 or PMS2 was transfected in parallel and visualized after 48 h using FITC-labeled goat anti-mouse IgG Alexa Fluor 488 for MLH1 detection (shown in green; left column)...

2007年2月2日 · The figure shows that expression of the TAP-tagged variants of MLH1 and PMS2 restored MMR proficiency and cell cycle checkpoint activation to the MMR-deficient cell lines 293T and HeLa12.

2023年4月24日 · To evaluate the effect of MLH1–PMS2 in rescuing DNA MMR activities in 293T cells, we co-transfected the pCAR-OF plasmid with different amounts of Flag–MLH1–PMS2 using the GFP plasmid as an internal standard.

PMS2 Knockout HEK293T Cells (PMS2 基因敲除HEK293T 细胞) 是通过同时表达Cas9 、目的基因sgRNA 和puromycin抗性基因,并实现了目的基因CRISPR 敲除的HEK293T 细胞。

2019年4月4日 · The FLAG-tagged MLH1–PMS2, 4KR-PMS2 or 4KQ-PMS2 was stably transfected into 293T cells, followed by 50 μ m 6-TG treatment for indicated time intervals. Anti-PARP1 and anti-β-actin Western blotting analyses were performed.

PMS2 293T Cell Transient Overexpression Lysate(Denatured) 2 Images Go to Product page Overview

Transient overexpression cell lysate was tested with anti-PMS2 antibody (Mfr. No. H00005395-D01P) by Western Blot.