Human embryonic kidney fibroblast 293T cells lacking expression of intrinsic MLH1 because of MLH1 promoter methylation were cotransfected with a pSG5 expression vector containing a …

Feb 24, 2003 · In this study, we have uncovered an interaction between PMS2 and p73. Furthermore, we have found that PMS2 can collaborate with p73 to enhance cisplatin-induced …

Aug 1, 2024 · HEK-293T cells were transfected with MLH1-HA in the absence or presence of PMS2-Flag. Cells were then collected at indicated time points after CHX treatment for …

May 1, 2004 · We exposed the 293T Lα + and 293T Lα − cells to ionizing radiation and monitored their viability and progress through the cell cycle for 72 h. Ionizing radiation arrested both cell …

We show here that the PMS2(R20Q) variant is defective in activat-ing p73-dependent apoptotic response to cisplatin. When ex-pressed in Pms2-deficient mouse fibroblasts, human …

As a control, unlabeled MLH1 or PMS2 was transfected in parallel and visualized after 48 h using FITC-labeled goat anti-mouse IgG Alexa Fluor 488 for MLH1 detection (shown in green; left …

Apr 24, 2023 · To evaluate the effect of MLH1–PMS2 in rescuing DNA MMR activities in 293T cells, we co-transfected the pCAR-OF plasmid with different amounts of Flag–MLH1–PMS2 …

Jan 31, 2012 · In this study, we demonstrate that miRNAs and MLH1 participate in a regulatory feedback loop, in which miRNA-422a negatively regulates the expression of MLH1 protein and …

Feb 2, 2007 · In this study, we used MLH1-deficient 293T cells (34) for transfection with TAP-tagged MLH1 and PMS2-deficient HeLa12 cells (35) for transfection with TAP-tagged PMS2. …

Apr 4, 2019 · The FLAG-tagged MLH1–PMS2, 4KR-PMS2 or 4KQ-PMS2 was stably transfected into 293T cells, followed by 50 μ m 6-TG treatment for indicated time intervals. Anti-PARP1 …