2014年9月3日 · Here we use whole-genome resequencing of six 293 cell lines to study the dynamics of this aneuploid genome in response to the manipulations used to generate common 293 cell derivatives, such as...

2022年8月1日 · Here, we analyzed transcriptome changes in HEK 293T cells after depletion of each DNA-PK subunit. Depletion of various DNA-PK subunits resulted in dramatic differences in the number of differentially expressed genes: only 7 genes changed more than 2-fold in DNA-PKcs-deficient cells, 29 genes in Ku80-deficient, 219 genes in Ku70-deficient.

2016年9月20日 · In this study, we present the first comprehensive analysis of the protein, messenger RNA and microRNA profiles of 293T cell-derived exosomes; then, we characterized these data using Gene Ontology annotation and Kyoto Encyclopedia for Genes and Genomes pathway analysis.

2023年5月16日 · In this work, the mRNA expression profile of three HEK-293 cell lines, resulting in various yields during a fermentation batch process for rAAV production, was investigated to gain basic insight into cell variability and eventually …

2022年12月24日 · HEK 293T cells treated with LNPs containing mRNA were able to access nutrients and produce protein for more than 24 h implying that the inevitable release of pseudouridine upon mRNA degradation...

2022年5月20日 · HEK-293人胚肾细胞：是用5型腺病毒75株系转化，含有Ad5 E1区的人胚肾亚三倍体细胞系，是一种E1区缺陷互补细胞系。 它是加拿大McMaster University 的F.L.Graham与J.S.Miley于1976年用DNA转染技术构建而成。 该细胞为人类腺病毒载体扩增的宿主。 可表达异常的玻连蛋白的细胞表面受体，由整合素β1亚单位和玻连蛋白受体α-v亚单位组成。 HEK-293T人胚肾细胞：人胚肾细胞293细胞插入SV40 T-抗原基因后产生的高转染效率的衍生株称为293T。 …

Compare the variations of 6 HEK293-cell-lines with each other and the reference sequence. The HEK293 (Human Embryonic Kidney 293) cell line is one of the most frequently used cell lines …

We examined the expression profiles of lncRNAs and mRNAs in 293T cells with or without 8 Gy irradiation using high-throughput RNA sequencing. We then performed comprehensive transcriptomic and bioinformatic analyses of these sequencing results.

SHENTEK® 293T 总RNA 残留检测试剂盒适用于定量检测生物制品中残留的293T细胞总RNA; 通过设计特异性引物和探针, 将反转录和荧光探针qPCR 检测技术融合,实现一步法定量检测总RNA 残留; 其检测快速, 专一性强, 性能可靠。

In the present study, to explore whether RIG-I is the major receptor for IBV to active the antiviral innate immune response and its effect on IBV replication, RIG-I gene in 293T cells was knocked out by CRISPR-Cas9 system, and a stable RIG-I knockout 293T (RIG-I-/- 293T) cell line was screened by puromycin pressure.