To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1.

2009年5月5日 · Coordinates and structure factors for both crystal forms have been deposited in the Protein Data Bank under ID codes 2WB1 (Crystal_1) and 2WAQ (Crystal_2). Sequencing of RNA polymerase subunits. Open reading frames for the S. shibatae RNAP subunits were amplified by PCR from genomic DNA using Pfu DNA polymerase prior to cloning in PCR script ...

The 13-subunit crystal structure of RNAP from the crenarchaeal species Sulfolobus shibatae (PDB: 2WAQ) . Favorable interactions with the promoter and basal TFs are eventually overcome permitting promoter escape [ 37 ] and before ternary elongation complexes (TECs) transcribe more than ~50 nts, most TECs exchange contacts with basal initiation ...

2015年1月1日 · The X-ray structure of the archaeal polymerase of S. shibatae 37 (PDB: 2WAQ) is represented as dark grey ribbon. Note, that at this confidence level, the credible volume of tDNA(-9) is divided into two areas, if the credible volume is drawn at higher confidence these two areas merge (see text for details).

Biological Unit for 2WAQ: tridecameric; determined by author and by software (PQS)

2009年5月19日 · 2waq: Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.

RNA polymerases catalyse the DNA dependent polymerisation of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). This domain, domain 2, contains the active site. The invariant motif -NADFDGD- binds the active site magnesium ion [1,2].

2WAQ: The complete structure of the archaeal 13-subunit DNA-directed RNA Polymerase

2waq is a 10 chain structure with sequence from Saccharolobus shibatae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.

2012年7月30日 · First, we solved the apo-aRNAP structure by molecular replacement technique in Phaser using the previous published aRNAP model (PDB ID 2WAQ). The refinement to 3.2 Å resolution was carried out using Phenix version 1.6.2 ( 10 ) with tight X-ray/stereochemistry weight (0.01), secondary structure restraints, TLS and B-group.