2010年4月9日 · Here we describe the crystal structure of the Tat.P-TEFb complex containing HIV-1 Tat, human Cdk9 (also known as CDK9), and human cyclin T1 (also known as CCNT1). Tat adopts a structure complementary to the surface of P-TEFb and makes extensive contacts, mainly with the cyclin T1 subunit of P-TEFb, but also with the T-loop of the Cdk9 subunit.

2010年6月10日 · Atomic coordinates and structure factors for Tat·P-TEFb and Tat·P-TEFb·ATP structures have been deposited in the Protein Data Bank with accession numbers 3mi9 and 3mia, respectively.

2017年2月8日 · The structural fluctuation of the Tat/CycT1/CDK9 tri-molecular complex (3MI9) was analyzed using Desmond ver. 3.8 as described in Experimental Procedures for 100 ns (Fig 1, left panel). The fluctuation of the CycT1/CDK9 bi-molecular structure was similarly examined using the coordinates of 3MI9 without the Tat protein moiety as the starting ...

cl08273 (2-64): The retroviral Tat protein binds to the Tar RNA. This activates transcriptional initiation and elongation from the LTR promoter. Binding is mediated... * Click molecule labels to explore molecular sequence information. Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH.

2010年6月10日 · Here we describe the crystal structure of the Tat.P-TEFb complex containing HIV-1 Tat, human Cdk9 (also known as CDK9), and human cyclin T1 (also known as CCNT1). Tat adopts a structure complementary to the surface of P-TEFb and makes extensive contacts, mainly with the cyclin T1 subunit of P-TEFb, but also with the T-loop of the Cdk9 subunit.

2010年6月9日 · 3mi9 overview; Citations; Structure analysis; Function and Biology; Ligands and Environments; Experiments and Validation; View

3MI9: Crystal structure of HIV-1 Tat complexed with human P-TEFb

Here we describe the crystal structure of the Tat.P-TEFb complex containing HIV-1 Tat, human Cdk9 (also known as CDK9), and human cyclin T1 (also known as CCNT1). Tat adopts a structure complementary to the surface of P-TEFb and makes extensive contacts, mainly with the cyclin T1 subunit of P-TEFb, but also with the T-loop of the Cdk9 subunit.

PDB Entry - 3MI9 (Status - Released) Summary information: Title: Crystal structure of HIV-1 Tat complexed with human P-TEFb. PDB DOI: https://doi.org/10.2210/pdb3mi9/pdb. Primary publication DOI: https://doi.org/10.1038/nature09131. Entry authors: Tahirov, T.H., Babayeva, N.D., Varzavand, K., Cooper, J.J., Sedore, S.C., Price, D.H.

为克服这一局限性，本研究提出了一种全局对接的方法，以受体蛋白为球状系统的中心，将多肽平均地分布在球面26个位置上；同时定义了一个区分天然结合构象和非天然结合构象的筛选参数。 用上述方法预测多肽–蛋白质的结合构象，结果显示该方法能成功预测蛋白质的结合位点，且多数多肽的预测构象的C α -RMSD在5.5 Å以下。 因此，研究结果表明，所发展的方法在蛋白质多肽结合位点预测方面有很好的应用价值。 多肽–蛋白质相互作用，多肽结合位点，分子对接. 1. 引言. …