Previous reports have shown that excess calorie intake promotes p53 dependent senescence in mouse adipose tissues. The objective of the current study was to address the mechanism …

2021年12月1日 · Aging is characterized by the accumulation of senescent cells in tissues, with an irreversible stop in the replicative cycle, resistance to apoptosis, and an altered pattern of molecules...

2009年6月13日 · We used an in vitro model to evaluate the effects of cellular aging and inflammation on the gene expression and protein secretion profiles of adipocytes. 3T3-L1 mouse preadipocytes were cultured according to standard conditions and analyzed at different time points both at the basal state and after an acute stimulation with LPS.

2024年6月1日 · Aging and obesity induce similar AT functional and structural changes, including an accumulation of senescent cells. To study the effect of Cu-mediated stress-induced premature senescent (Cu-SIPS) on preadipocytes, 3T3-L1 cell line was exposed to a subcytotoxic concentration of copper sulfate.

2024年8月20日 · In this study, we evaluated the role of MBL in oxidative stress-induced premature aging and explored its underlying mechanism in C57BL/6 mice and mouse embryonic fibroblasts (NIH/3T3). First, we established an oxidative premature senescence model induced by D-galactose in C57BL/6 mice.

2019年7月1日 · In our in vitro culture of 3T3-L1 aging adipocytes, the percentage of senescent cells, as evaluated by SA-β-gal staining, increases significantly with the aging of the culture; aged cultures at PID18 and PID26 present a significant higher degree of senescent cells than younger cultures at PID10 (Fig. 1 -A, C).

2016年2月5日 · 因此本文利用小鼠胚胎成纤维细胞系 NIH／3T3 细胞，建立 H 2 O 2 诱导的经典的细胞衰老模型。 MicroRNAs（miRNAs，微小 RNA）是近年来涌现的一类强大的基因表达调控子，可通过对下游靶基因表达的负调控，广泛参与多种生命进程，在细胞衰老过程中同样扮演着至关重要的角色[3]。 miR －33 家族由固醇调节元件结合蛋白基因（SREBP）的转录本中剪切加工而来，可参与脂质代谢的调节，并与老年性疾病如动脉粥样硬化的发生密切相关[4 －6] 。 近来的 …

The data are consistent with the observation that metformin has a protective effect in this in vitro model of aging 3T3 fibroblasts under high glucose conditions inducing cell proliferation, collagen I and III production, protection from apoptosis, and reducing NF-kB (p65) activity.

These data suggest that aging adipocytes in vitro show a decline in pro-adipogenic signals, in genes involved in glucose metabolism and cytoskeleton maintenance and in adiponectin. These changes are paralleled by an increase in inflammatory cytokines; inflammation seems to mimic and amplify the effects of cellular aging on adipocytes.

2020年10月10日 · Using cultured 3T3-L1 cells, we investigated the involvement of energy regulators Sirt1, AMPK, and LKB1 in senescence. Fifteen days post differentiation, Sirt1 knock-down increased senescence-associated beta-galactosidase (SA-β-Gal) staining by 20-40% (p<0.05, n=12) and both cyclin kinase inhibitor p21 Cip and chemokine receptor IL8Rb ...