广泛的生化、结构和遗传分析表明，43s pic在多蛋白eif4f复合物（eif4a、eif4e和eif4g）促进的封端mrna的5‘末端附近结合，并通过5‘非翻译区（5’utr）定向移动，以搜索第一个遇到的起始密码子，这一过程称为扫描。

2022年11月23日 · Extensive biochemical, structural, and genetic analyses suggest that the 43S PIC binds near the 5′ end of a capped mRNA facilitated by a multi-protein eIF4F complex (eIF4A, eIF4E, and eIF4G) and moves directionally through the 5′ untranslated region (5′ UTR) in search of the first encountered start codon, in a process called scanning ...

eif3可以与40 s小亚基的多个位点结合，环绕整个小亚基，还可与多种起始因子相互作用，募集并调节其精细定位，从而形成多因子复合物（mfc）。 酵母和兔的40 S亚基与eIF3复合物。

Eukaryotic initiation factor 4E (eIF4E) has long been known as the cap-binding protein that participates in recruitment of mRNA to the ribosome. A number of recent advances have not only increased our understanding of how eIF4E acts in translation but …

40S 粒子的有效募集对于编码癌蛋白和生长促进因子的 mRNA 的翻译尤为重要，这些 mRNA 通常包含复杂的 5' UTR，需要有效启动。 因此，抑制 eIF4E/eIF4G 相互作用已成为开发抗癌剂的先前未探索的途径。 事实上，我们发现了这种 eIF4E/eIF4G 相互作用 (4EGI) 的小分子抑制剂，可在体外和体内抑制翻译起始，并已成功用于许多癌症生物学和神经生物学研究。 然而，它们详细的分子作用机制仍然难以捉摸。 在这里，我们展示了 eIF4E/eIF4G 抑制剂 4EGI-1 通过与 eIF4E 上 …

Eukaryotic initiation factor 4E (eIF4E) binds the mRNA cap structure and forms eIF4F complexes that recruit 40S subunits to the mRNA. Formation of eIF4F is blocked by eIF4E-binding proteins such as 4E-BP1, which interacts with eIF4E via a motif in ...

2024年5月1日 · Messenger RNA (mRNA) recruitment to the 40S ribosomal subunit is mediated by eukaryotic initiation factor 4F (eIF4F). This complex includes three subunits: eIF4E (m 7 G cap-binding protein), eIF4A (DEAD-box helicase), and eIF4G. Mammalian eIF4G is a scaffold that coordinates the activities of eIF4E and eIF4A and provides a bridge to connect the ...

2024年1月29日 · eIF4E stabilizes the binding of mRNA to eIF4G–eIF4A 36 and stimulates the helicase activity of the eIF4F bound eIF4A 69. Selective 40S ribosome profiling has indicated that scanning is cap ...

2016年7月1日 · Ribosomal attachment to mammalian capped mRNAs is achieved through the cap-eukaryotic initiation factor 4E (eIF4E)-eIF4G-eIF3-40S chain of interactions, but the mechanism by which mRNA enters the mRNA-binding channel of the 40S subunit remains unknown. To investigate this process, we recapitulated i …

2024年4月4日 · Messenger RNA (mRNA) recruitment to the 40S ribosomal subunit is mediated by eukaryotic initiation factor 4F (eIF4F). This complex includes 3 subunits: eIF4E (m 7 G cap binding protein), eIF4A (DEAD-box helicase), and eIF4G. Mammalian eIF4G is a scaffold that coordinates the activities of eIF4E and eIF4A and provides a bridge to connect the ...