Top Features of Incucyte ® Caspase-3/7 Dye for Apoptosis . Reduce loss of precious or compromised cells under-going caspase-3/7 mediated apoptosis with mix-and-read reagents; Track true, artifact-free biological responses with non-perturbing, live-cell reagents; Quantify apoptosis in real-time with the Incucyte ® Live-Cell Analysis System

CellEvent Caspase-3/7 Detection Reagents are a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. The DEVD peptide sequence is a cleavage site for caspase-3/7, and the conjugated dye is non-fluorescent until cleaved from the peptide and bound to DNA.

本检测法强大、稳定，能够用于多种荧光研究平台的高通量分析中，例如微孔板分析，免疫组化和流式细胞术。 使用时96孔板时，每孔用100ul试剂，本试剂盒提供的试剂足够进行200次检测；用384微孔板时，每孔加25ul试剂，本试剂盒提供的试剂足够进行800次检测。 细胞凋亡检测. 详见说明书. Component A: Caspase 3/7 Substrate (200X …

2011年1月8日 · The Incucyte® Caspase-3/7 Dyes for Apoptosis couple the activated caspase-3/7 recognition motif (DEVD) to a DNA intercalating dye and are ideally suited to the mix-and-read, real-time quantification of cells undergoing caspase-3/7 mediated apoptosis.

Incucyte® Caspase-3/7 染料 为惰性、非荧光 (DEVD) 的底物，可自由穿过细胞膜，它们可被激活的 Caspase-3/7 裂解，释放出绿色或红色可结合 DNA 的荧光标记。 通过荧光标记的细胞核的出现来识别凋亡细胞。 Incucyte® Annexin V 染料 标记有异常明亮且具有光稳定性的 CF 染料，在与凋亡细胞中暴露的 PS 结合后，发出绿色、红色、橙色或近红外荧光信号。 ® ® ®. 了解更多信息. 查看 Incucyte ® 细胞凋亡的相关文献. 图 1. 使用 Incucyte® 细胞凋亡分析和图像分析工具对选 …

Generate data rich information within a single well using fluorescent reagent combinations in adherent or non-adherent cell cultures. Investigate a range of cell models (mono-, co- or tri-culture in 2D or 3D) using lab-tested, validated protocols to answer relevant scientific questions faster.

Incucyte ® Apoptosis Assays allow every well of a 96/384 well plate to be imaged and analyzed automatically to provide a microplate readout of cell death over time (A). Time-courses reveal concentration-dependent treatment effects (B).

Incucyte Caspase-3/7 dye (Sartorius, #4440) was added to the co-cultures to determine target cell apoptosis induced by NK cells or CD8 T cells.

The Incucyte® Caspase-3/7 Dyes for Apoptosis couple the activated caspase-3/7 recognition motif (DEVD) to a DNA intercalating dye and are ideally suited to the mix-and-read, real-time quantification of cells undergoing caspase-3/7 mediated apoptosis.

The Incucyte Caspase-3/7 Green Dye is a fluorescent reagent designed for real-time monitoring of apoptosis in live cells. It binds to activated caspase-3/7 enzymes, which are key mediators of the apoptotic process.