The BD Cytofix/Cytoperm Plus Fixation/Permeabilization Kit provides Fixation/Permeabilization solution, BD Perm/Wash™ Buffer, and BD GolgiStop™ protein transport inhibitor containing monensin. The ex vivo addition of BD GolgiStop™ to in vitro- or in vivo-stimulated cells blocks their intracellular transport processes.

BD Cytofix/Cytoperm™ solution is supplied as a 1X solution and can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining. Store undiluted at 4°C. For the stimulation of cells, various in vitro methods have been reported for stimulating cells to produce cytokines.

The BD Cytofix/Cytoperm Plus Fixation/Permeabilization Kit provides Fixation/Permeabilization solution, BD Perm/Wash™ Buffer, and BD GolgiStop™ protein transport inhibitor containing monensin. The ex vivo addition of BD GolgiStop™ to in vitro- or in vivo-stimulated cells blocks their intracellular transport processes.

The ex vivo addition of GolgiStop to in vitro- or in vivo -stimulated cells blocks their intracellular transport processes. This results in the accumulation of most cytokine proteins in the Golgi complex and thereby enhances cytokine staining signals. Spot an opportunity for improvement? Share a Content Correction.

Fixation and Permeabilization Solution Kit with BD GolgiStop™ (Cat. No. 554715) Kit components: In addition to the fixation/permeabilization and diluent/wash solutions included in the BD Cytofix/Cytoperm Kit, this kit includes BD GolgiStop, …

BD Cytofix/Cytoperm Plus 固定/破膜试剂盒提供固定/破膜溶液、BD Perm/Wash缓冲液和含有莫能菌素的 BD GolgiStop蛋白质转运抑制剂。 将 BD GolgiStop 体外添加到体外或体内刺激的细胞中可阻断其细胞内转运过程。 这会导致大多数细胞因子蛋白在高尔基复合体中积累，从而增强细胞因子染色信号。 提供的 BD GolgiStop 试剂足以处理高达 1 升的细胞培养物，细胞密度高达 2 × 10 ⁶细胞/毫升. 成分 数量 固定/透化溶液 125 mL BD Perm/Wash 缓冲液 (10x) 100 mL BD …

此类激活剂主要包括以下物质：佛波酯加钙离子载体或离子霉素，植物凝血酶，葡萄球菌肠毒素 B， 以及直接针对 TCR/CD3 复合物的单克隆抗体（加或不加针对共刺激受体如 CD28 的抗体） f注意：已报道单独的 PMA 刺激会导致小鼠 T 细胞表面 CD4 的表达降低。 PMA 与钙离子载体共同. 刺激则使 CD4 表达降低更多，同时也会导致小鼠 CD8 胸腺细胞及 小鼠和人外周血 T 淋巴细胞减少。 1.）使用 BD GolgiStop™蛋白转运抑制剂（含莫奈霉素）的操作步骤 每 6ml 细胞培养基 …

2024年4月9日 · 丁香通为您提供BD PharmingenCytofix/Cytoperm W/Golgi Stop Kit价格等详细信息，产品供应商为上海远慕生物科技有限公司，产品类别为抗体，货号是554715。 如需批量采购，还请访问丁香通官网。

The BD Cytofix/Cytoperm Plus Fixation/Permeabilization Kit provides Fixation/Permeabilization solution, BD Perm/Wash™ Buffer, and BD GolgiStop™ protein transport inhibitor containing monensin. The ex vivo addition of BD GolgiStop™ to in vitro- or in vivo-stimulated cells blocks their intracellular transport processes.