2015年10月6日 · Additional measurement also revealed that different EGFR positive cell lines (A431, HeLa, and A549) show receptor density dependent anti-EGFR binding kinetics. The results demonstrate that SPRi is a valuable tool for direct quantification of membrane protein expression level and ligand binding kinetics at single cell resolution.

A431 cells are used in studies of the cell cycle and cancer-associated cell signalling pathways since they express abnormally high levels of the Epidermal growth factor receptor (EGFR). They are often used as a positive control for EGFR expression .

2013年4月26日 · The ability of EGF bioconjugates to target the EGF receptors was tested using EGFR over-expressing A431 cells in comparison to EGFR negative HeLa cells. Our results showed that the bioconjugates where the EGF was bonded by carbodiimide chemistry are the most effective for the specific targeting of EGFR-expressing cells in vitro.

2021年9月3日 · Therefore, biparatopic nanobodies and its ADC, equipped with two different papratopes and two modes of EGFR inhibition, could exert potent dose-dependent antitumor activity in A431 tumor...

The tyrosine kinase inhibitor GEF has been reported to induce dimer formation by enhancing the affinity of EGF-EGFR, while lapatinib stabilizes the inactive form of EGFR and reduces the...

A431 cells which express 4- to 5-fold more EGF receptors than A431-4 cells were growth inhibited, while A431-4 cells were growth stimulated by EGF within the same dose range. Treatment of A431 cells with EGF resulted in a 2-fold increase in cellular IP3 levels and the effect in A431-4 cells was not as obvious.

2023年12月14日 · To validate the performance of EGFR-FabID, we compared five cell lines with different EGFR expression levels: A431 (high), Expi293F-EGFR stable (middle), HeLaS3 (low), NCI-H1975 (low), and...

Epidermal growth factor (EGF) at 3 nM maximally inhibits the proliferation of A431 epidermoid carcinoma cells. We show that at lower concentrations, in the range of 3-100 pM, EGF has a mitogenic effect on A431 cells. In the presence of 100 nM anti-EGF-receptor monoclonal IgG (designated 528), which …

2003年9月1日 · To study the phosphorylation on each individual site, EGFR generated from a human epidermoid carcinoma cell line (A431) was analyzed by mass spectrometry. Liquid chromatography combined with tandem mass spectrometry (LC/MS/MS) was used to identify the tryptic phosphopeptides and their sites of phosphorylation (Y992, Y1045, Y1068, Y1086, S1142 ...

Additional measurement also revealed different EGFR positive cell lines (A431, HeLa and A549) show receptor density dependent anti-EGFR binding kinetics. The results demonstrate that SPRi is a valuable tool for direct quantification of membrane protein expression level and ligand binding kinetics at single cell resolution.