基因沉默shRNA载体设计与构建 - 知乎 - 知乎专栏
研究中采用的重组腺相关病毒载体（Recombination adeno-associated virus, rAAV）是在非致病的野生型AAV的基础上改造的基因载体，具有种类多样、免疫原性低、安全性高、宿主范围广、 …
scAAVengr, a transcriptome-based pipeline for quantitative …
2021年10月19日 · Öztürk et al. addressed this issue by developing an experimental pipeline called scAAVengr for comparing gene therapy vectors head-to-head. The process involves …
Quantitative single-cell transcriptome-based ranking of …
2022年6月9日 · In this study, we evaluated 18 wild-type and engineered AAV capsids in human retinal explants using a recently developed single-cell RNA sequencing (RNA-seq) AAV …
Spatial transcriptomics for profiling the tropism of viral vectors in ...
2023年1月26日 · To address these limitations, we recently reported a method based on single-cell RNA sequencing (scRNA-seq) that enables deep characterization of pooled AAV variants in …
哺乳动物shRNA干扰AAV载体-载体百科 | 云舟生物
我们的腺相关病毒（adeno-associated virus，简称AAV）shRNA干扰载体系统是一种非常高效的能稳定在体内和体外干扰各种哺乳动物细胞靶基因表达的载体工具。AAV的免疫原性极低，在体 …
哺乳动物shRNA干扰AAV载体—云舟生物 - 知乎 - 知乎专栏
我们的 腺相关病毒 （adeno-associated virus，简称AAV） shRNA干扰载体 系统是一种非常高效的能稳定在体内和体外干扰各种哺乳动物细胞靶基因表达的载体工具。AAV的免疫原性极低， …
A comprehensive analysis of scRNA-Seq and RNA-Seq unveils B
2025年3月5日 · In this study, transcriptomic and scRNA-seq data are employed to investigate the causal relationships between immune factors and the brain immune environment associated …
AAV-mediated expression of proneural factors stimulates …
2025年3月6日 · (B) UMAP of integrated scRNA-seq data from AAV-borne Ascl1 and control vectors clustered by cell type. (B’) Feature plots of subset reprogrammed clusters showing the …
Massively parallel in vivo Perturb-seq screening - Nature
2025年2月12日 · Here we describe a detailed protocol to perform massively parallel in vivo Perturb-seq using several versatile adeno-associated virus (AAV) vectors and provide …
实现高效基因沉默：AAV介导的shRNA克隆技术概览 | 派真生物
在遗传研究和基因治疗领域，小干扰RNA (shRNA)是一项革命性的技术，可以精确地靶向并沉默特定的基因表达。 利用腺相关病毒 (AAV)作为载体进行shRNA克隆，是一种有效的基因递送方 …