BMSC osteogenic differentiation was detected by ALP staining, Alizarin Red solution staining and RT-PCR. ACP NPs were safe to macrophages but promoted autophagy and induced ROS production at high concentrations. ACP NPs changed morphology of macrophages and induced polarization into M1 type, thus promoting the expression of inflammatory cytokines.

2020年9月16日 · BMSC osteogenic differentiation was detected by ALP staining, Alizarin Red solution staining and RT-PCR. ACP NPs were safe to macrophages but promoted autophagy and induced ROS production at high concentrations. ACP NPs changed morphology of macrophages and induced polarization into M1 type, thus promoting the expression of inflammatory cytokines.

2021年12月1日 · Our results revealed that icariin treatment/miR-23a knockdown promoted BMSC viability and osteogenic differentiation as well as increased the mRNA and protein expression of BMP-2, BMP-4, Runx2, p-Smad5, Wnt1 and β-catenin in BMSCs and ONFH model rats.

Our results revealed that icariin treatment/miR-23a knockdown promoted BMSC viability and osteogenic differentiation as well as increased the mRNA and protein expression of BMP-2, BMP-4, Runx2, p-Smad5, Wnt1 and β-catenin in BMSCs and ONFH model rats.

Rat bone marrow stromal cells (BMSC) were cultured for 21 days in osteogenic medium and mineral was added to cell layers at 4, 11, 18 days. β-glycerophosphate was excluded from osteogenic medium to allow for more rigorous assessment of the effect of ion release from ACP.

2020年2月24日 · Objective: To evaluate macrophage responses to ACP nanoparticles and the effect of ACP nanoparticle/macrophage-modulated environments on the osteogenic ability of BMSCs. Materials and methods:...

2021年2月1日 · BMSC osteogenic differentiation was detected by ALP staining, Alizarin Red solution staining and RT-PCR. ACP NPs were safe to macrophages but promoted autophagy and induced ROS production at...

ACP NPs/macrophage-modulated environments weaken the osteogenic ability of BMSCs. ACP NPs may cause aseptic inflammation and attenuate osteogenesis. KEY WORDS: ACP nanoparticles; macrophage polarization; cytokine; autophagy; osteogenic differentiation. INTRODUCTION Calcium phosphate (CaP) is often used as a bone substitute or coating material.

BMSC osteogenic differentiation was detected by ALP staining, Alizarin Red solution staining and RT-PCR. ACP NPs were safe to macrophages but promoted autophagy and induced ROS production at high concentrations. ACP NPs changed morphology of macrophages and induced polarization into M1 type, thus promoting the expression of inflammatory cytokines.

摘要： 目的比较ScienCell (SCI)干细胞培养基和低糖 (LG)完全培养基培养下骨髓间充质干细胞 (BMSC)的免疫学特性。...