
AFM 用于DNA分子扫描成像的实验,着重于样 品制备技术和实验步骤.该实验既能让学生掌握 AFM 的原理,又能让他们学习一些基本的AFM 扫描用生物样品制备方法. 2 实验原理 2.1 装置原理 AFM 利用微悬臂上的探针尖端充当力传感
科研干货 | 原子力显微镜AFM生物样品制样方法 - 知乎
原子力显微镜afm作为一种重要的单分子研究工具,在教学、科研领域的研究中得到了广泛应用。 目前主要以测量普通样品的形貌为主,因为生物样品十分“娇贵”,制样方法比较繁琐。
Atomic Force Microscopy of DNA and DNA-Protein Interactions
2022年5月31日 · Atomic force microscopy (AFM) is a microscopy technique that uses a sharp probe to trace a sample surface at nanometer resolution. For biological applications, one of its key advantages is its ability to visualize the substructure of single molecules and molecular complexes in an aqueous environment.
Frontiers | DNA studies using atomic force microscopy: …
2015年1月28日 · A complementary approach, the imaging-based technique using Atomic Force Microscopy (AFM) provides direct visualization and measurement of individual DNA fragments. In this review, we summarize and discuss the application of …
AFM for analysis of structure and dynamics of DNA and protein–DNA ...
2009年3月1日 · This paper describes protocols for studies of structure and dynamics of DNA and protein–DNA complexes with atomic force microscopy (AFM) utilizing the surface chemistry approach. The necessary specifics for the preparation of functionalized surfaces and AFM probes with the use of silanes and silatranes, including the protocols for synthesis ...
DNA at conductive interfaces: What can atomic force microscopy …
2023年6月1日 · This review is devoted to the analysis of the capabilities of atomic force microscopy (AFM) in the study of the behavior of DNA at conducting solid–liquid and solid-air interfaces under electrochemical control and after the influence of the potential differences.
Atomic force microscopy—A tool for structural and translational DNA …
2021年7月9日 · Atomic force microscopy (AFM) is a powerful imaging technique that allows for structural characterization of single biomolecules with nanoscale resolution. AFM has a unique capability to image biological molecules in their native states under physiological conditions without the need for labeling or averaging.
Imaging DNA Structure by Atomic Force Microscopy
2016年6月10日 · Here, we describe two methods for in-liquid AFM imaging of DNA that have been successfully employed to visualize the DNA double helix : the widely implemented amplitude modulation mode (also called tapping, intermittent contact, or AC mode); and the rapid force-distance imaging mode (also called PeakForce tapping or Quantitative Imaging mode ...
Imaging of DNA and Protein–DNA Complexes with Atomic Force Microscopy
The advent of the atomic force microscopy (AFM) nanoimaging technique heralded new molecular biology applications for the study of DNA and protein–DNA complexes. A number of features make AFM a valuable technique in biological studies, including gentle sample preparation techniques, which minimize concerns about sample preservation typically ...
一文读懂原子力显微镜(AFM)在生物中的应用及常见测试问答 - 知乎
液态环境中应用 AFM 技术对 DNA 片段进行检测,并记录了运动轨迹。 图 1. DNA 分子折纸上的轨道运动. AFM 不仅能够提供超光学极限的细胞结构图像,还能够探测细胞的微机械特性。 利用 AFM 还能够对固定的活细胞和亚细胞结构进行深入研究,这些研究获得了有关细胞器的构造、细胞膜和细胞骨架等更详细的信息,同时将细胞固定在基底上再进行 AFM 观察,还可以得到层状脂肪物、微端丝和微绒毛等特征。 图2. 细胞表面微绒毛变化. Q1: 激光共聚焦显微镜 、 扫描电镜 …