2024年8月1日 · Aldo-keto reductases (AKRs) constitute a superfamily of oxidoreductases that catalyze the reduction of aldehydes and ketones to their corresponding alcohols using NAD (P)H as a coenzyme. Knowledge about the crucial roles of AKRs immobilization in the biocatalytic synthesis of chiral alcohols is expanding.

In this study, the SpyTag/SpyCatcher binding pair was fused to the N-terminus of an alcohol dehydrogenase (ADH) and an aldo-keto reductase (AKR), respectively. A non-canonical amino acid (ncAA), p -azido-L-phenylalanine (p-AzF), as the anchor for covalent bonding enzymes, was genetically inserted into preselected sites in the AKR and ADH.

2022年4月30日 · When aldehyde ketone reductase (AKR), alcohol dehydrogenase (ADH) and glucose dehydrogenase (GDH) were utilized as model enzymes, their mutants containing p -azido- L -phenylalanine were bio-orthogonally crosslinked with diyne to form crosslinked dual enzymes (CLDEs) acting as a cascade biological oxidation and reduction system.

2024年3月8日 · Aldo-keto reductases (AKRs) are a superfamily of oxidoreductases capable of reducing carbonyl to primary and secondary alcohols, found in all phyla 22.

2024年5月27日 · In this study, the SpyTag/SpyCatcher binding pair was fused to the N-terminus of an alcohol dehydrogenase (ADH) and an aldo-keto reductase (AKR), respectively. A non-canonical amino acid (ncAA), p-azido-L-phenylalanine (p-AzF), as the anchor for covalent bonding enzymes, was genetically inserted into preselected sites in the AKR and ADH.

2024年8月12日 · Zn2+ 离子诱导最小蛋白质模块的聚集，例如 6-His 标签、富含脯氨酸的基序 （PRM） 和 SRC 同源 3 （SH3） 结构域，并允许偶联的醛酮还原酶 （AKR） 和醇脱氢酶 （ADH） 在低浓度下进行相分离。

2024年1月1日 · 研究者们通过将SpyTag/SpyCatcher结合对融合到醇脱氢酶（ADH）和醛酮还原酶（AKR）的N-末端，成功实现了多酶的有序共固定化。 利用非规范氨基酸p-azido-L-苯丙氨酸（p-AzF）作为酶的共价键合锚点，分别在AKR和ADH的预选位点上进行基因插入。

2018年7月16日 · Aldo–keto reductase (AKR) or alcohol dehydrogenase (ADH)-mediated stereoselective reduction of prochiral carbonyl compounds is an efficient way of preparing single enantiomers of chiral alcohols due to the high chemo-, enantio-, and regioselectivity of the enzymes (Ma et al. 2013).

Aldo-keto reductases (AKRs) are phase I metabolising enzymes that catalyse the reduced nicotinamide adenine dinucleotide (phosphate) (NAD (P)H)-dependent reduction of carbonyl groups to yield primary and secondary alcohols on a wide range of substrates, including aliphatic and aromatic aldehydes and ketones, ketoprostaglan-dins, ketosteroids and...

Herein, the SpyTag/SpyCatcher binding pair was fused to the N‐terminus of an alcohol dehydrogenase (ADH) and an aldo‐keto reductase (AKR), respectively. A non‐canonical amino acid (ncAA), i.e....