Human AP endonuclease (APE1), like most AP endonucleases, is of class II and requires an Mg 2+ in its active site in order to carry out its role in base excision repair. The yeast homolog of this enzyme is APN1.

除 DNA 修复活性外，APE 1 还能通过氧化还原机制（Ref-1）在体外调控多种转录因子的 DNA 结合活性。APE 1 已被证实能刺激 Fos-Jun 异源二聚体、Jun-Jun 同源二聚体、Hela 细胞 AP-1 蛋白以及包括 NF-kB、Myb 和 ATF/CREB 家族成员在内的其它几种转录因子的 DNA 结合活 …

摘要：脱嘌呤/脱嘧啶核酸内切酶1 (Apurinic/apyrimidinic endonuclease 1，APE1)是一种广泛存在于生物体内、在碱基切除修复 (Base excision repair，BER)过程中能够在碱基缺失位点 (AP site)处识别并切割DNA的蛋白酶，其作用效率高且特异性强。 同时，APE1在一些癌症细胞中的活性较正常细胞明显偏高，因此其自身也是一种癌症生物标志物。 目前，通过在DNA上人工设计AP位点，利用APE1的切割能力生成理想的功能核酸链，并结合不同的信号输出及放大方式，研究者 …

2022年9月14日 · Here, we determine how the base excision repair (BER) endonuclease AP-endonuclease 1 (APE1) recognizes and cleaves DNA damage in the nucleosome. Kinetic assays determine that APE1 cleaves...

APE1 is an essential enzyme in the base excision repair pathway of DNA lesions caused by oxidation and alkylation. As importantly, APE1 also functions as a redox agent maintaining transcription factors involved in cancer promotion and progression in an active reduced state.

Human apurinic/apyrimidinic endonuclease 1 (APE1) is the primary cellular apurinic/apyrimidinic (AP) endonuclease, playing a pivotal role in not only the removal and repair of numerous DNA lesions, but also as a redox activator of numerous transcription factors, including Egr1, NF-κB, p53, and HIF1a [1].

2015年10月12日 · DNA apurinic-apyrimidinic (AP) sites are prevalent noncoding threats to genomic stability and are processed by AP endonuclease 1 (APE1). APE1 incises the AP-site phosphodiester backbone,...

The mammalian AP-endonuclease (APE1/Ref-1) plays a central role in the repair of oxidized and alkylated bases in mammalian genomes via the base excision repair (BER) pathway. However, APE1, unlike its E. coli prototype Xth, has two unique and apparently distinct transcriptional regulatory activities. APE1 functions as a redox effector factor ...

2025年1月14日 · The enzyme apurinic/apyrimidinic endonuclease 1 (APE1) initiates repair of AP-sites by cleaving the DNA backbone at the AP-site via its endonuclease activity. Here, we investigated the functional role of the APE1 active site residue N174 that contacts the AP-site during catalysis.

Human apurinic/apyrimidinic endonuclease 1(APE1) is an essential BER protein that is involved in the processing of potentially cytotoxic abasic sites that are obligatory intermediates in BER. Here we provide a summary of the basic mechanistic role of APE1 in DNA repair and redox regulation and highlight preclinical and clinical data that ...