The Anti-ACSA-2 antibodies (ACSA-2: astrocyte cell surface antigen-2) have been developed for the detection of astrocytes from cell suspensions of mouse neural tissue based on the expression of the ACSA-2 antigen.

IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

The ACSA-2 antigen is specifically expressed on GLAST (ACSA-1) positive astrocytes and is therefore a specific marker of astrocytes in the developing and neonatal mouse central nervous system (CNS). The percentage of ACSA-2 positive astrocytes differs according to the mouse age and the brain region.

Compatible applications: IF, IHC Protocol: Protocol for MACSima™ Imaging Cyclic Staining Data sheet | ACSA-2 Antibody, anti-mouse, FITC, REAfinity™ V.05 | revised 30.SEP.2024 1/2

IHC: 适用于组织样本的石蜡 (IHC-p)或冰冻 (IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA (peptide): 适用于抗原肽的ELISA检测. ACSA Antibody detects endogenous levels of total ACSA. 引用格式: Affinity Biosciences Cat# DF3728, RRID:AB_2836092. Unconjugated.

ACSA Polyclonal Antibody detects endogenous levels of protein. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -15°C to -25°C/1 year (Do not lower than -25°C) 1 mg/ml. 77kD. Unmodified. Polyclonal. IgG. Synthesized peptide derived from human protein . at AA range: 40-120.

Anti-ACSA antibodies are used in antigen-specific immunodetection in biological samples. The target ACSA is a known alias name of the protein acyl-CoA synthetase short chain family member 2, encoded by the ACSS2 gene in humans.

ACSA-2是一种可商购获得的抗体，最初是使用磁性细胞分选方法开发的，用于从年轻的出生后的小鼠大脑中分离星形胶质细胞，但尚未公开其在从成年组织中分离细胞的实用性。 使用修改后的协议，我们现在显示该工具还可用于从成年大脑中分离超纯星形胶质细胞。 此外，我们使用了多种技术（包括单细胞测序，过表达和敲低分析，免疫印迹和免疫组织化学），首次将ACSA-2表位鉴定为ATP1B2，并表征了其在中枢神经系统中的分布。 最后，我们表明ATP1B2在中枢神经系统 …

Here we selected several cell surface antigens including Astrocyte Cell Surface Marker 2 (ACSA2), Cluster of differentiation 9 (CD9), lysophosphatidic acid receptor 1 (LPAR1), and Proteolipid protein 1 (PLP1) as potential markers of enteric glia.