最近发现GW182蛋白富含甘氨酸和色氨酸的基序（GW motif），它可以直接与人Argonaute蛋白的MID-PIWI区域相互作用，还发现由两个GW基序串联组成的名为“Ago钩（Ago hook）”的最小片段也能够完成这种相互作用。

2025年1月15日 · Argonaute proteins are categorized into AGO and PIWI clades. Across most animal species, AGO-clade proteins are widely expressed in various cell types, and regulate normal gene expression 1....

The evolutionarily conserved Argonaute/PIWI (AGO/PIWI; a.k.a. PAZ-PIWI Domain, or PPD) family of proteins is crucial for the biogenesis and function of small non-coding RNAs (ncRNAs). This family can be divided into AGO and PIWI subfamilies. The AGO proteins are ubiquitously present in diverse tissues.

2022年5月24日 · Prokaryotic Argonaute (pAgo) proteins are well-known oligonucleotide-directed endonucleases, which contain a conserved PIWI domain required for endonuclease activity. Distantly related to pAgos, PIWI-RE family, which is defined as PIWI with conserved R and E residues, has been suggested to exhibit divergent activities.

Argonaute (AGO)：最近的研究表明，PAZ结构域结合到 siRNA 的3’的二 核苷酸 突出端；一些AGO蛋白质的PIWI结构域赋予slicer以内切酶的活性。 PAZ和PIWI两个结构域，对于siRNA和目标 mRNA 相互作用，从而导致目标mRNA的切割或者翻译抑制过程，是必不可少的。

2014年11月19日 · AGO structure modelling and mutational analyses reveal that the QF-V motif within the conserved PIWI domain contributes to recognition of base pairing at the 15th nucleotide of a duplex, while...

2025年1月16日 · Argonaute 蛋白在进化过程中分成了两类——AGO 类和 PIWI 类。 在大多数动物物种中，AGO 类蛋白在多种细胞类型中广泛表达，并调控正常的基因表达。 相比之下，PIWI 类蛋白主要在配子发生过程中发挥作用，以抑制转座子并确保生殖能力。

The evolutionarily conserved Argonaute/PIWI (AGO/PIWI, also known as PAZ-PIWI domain or PPD) family of proteins is crucial for the biogenesis and function of small noncoding RNAs (ncRNAs). This family can be divided into AGO and PIWI subfamilies.

AGO protein family are evolutionarily conserved and consist of four domains: N terminus, PAZ, MID and PIWI. Based on the phylogenetic analysis and bound small RNAs, AGO family can be divided into three subfamilies: AGO-like, PIWI-like and Group3. All of the discovered plant AGO proteins are corresponded to the AGO-like subfamily.

作为RISC的核心成分，AGO蛋白(argonaute proteins)由N末端、PAZ、MID和PIWI 4个结构域组成. PAZ区能非序列特异性识别结合双链小RNA 3′末端悬垂的2个核苷酸，MID与PIWI界面处的“保守口袋”识别结合小RNA 5′端第1位核苷酸，PIWI区具有切割mRNA的催化中心.