2017年12月1日 · PI and GFP fluorescence were detected in root cells using respectively 488-nm and 580–620 nm (autof.) or 505–530 nm (GFP) as excitation and detection wavelengths in the laser confocal...

2024年1月31日 · We induced transcription of SHR–GFP in its endogenous expression domain (the central tissues of the root; Fig. 1a) using diminishing concentrations of dexamethasone 26 (dex) (10, 1, 0.05, 0.03 ...

2015年6月8日 · A CDF4-GFP (green fluorescence protein) protein fusion localizes to the nucleus (Figure S2), consistent with a role of CDF4 in transcriptional regulation. Whole-mount in situ hybridization shows that CDF4 is expressed in the upper differentiated columella cells and in the CSCs of wild-type roots, whereas no expression is detectable in the QC ...

2004年10月1日 · Here, we identify the PLETHORA1 (PLT1) and PLT2 genes encoding AP2 class putative transcription factors, which are essential for QC specification and stem cell activity. The PLT genes are transcribed in response to auxin accumulation and are dependent on auxin response transcription factors.

Auxin maximum co-localizes with the WOX5 expression domain in the quiescent center that separates mitotically active proximal and distal root meristems. Here we unravel the interconnected processes happening under WOX5 overexpression by combining in vivo experiments and mathematical modeling.

近年来出现的新型wtgfp基因突变体的激发和发射谱发生了改变，热稳定性和荧光强度得到了提高，gfp报告基因在小鼠中的应用就是以这些变体作为基础的。 （4）增强型绿色荧光蛋白（egfp） 现在，应用最为广泛的是红移变体增强型gfp（egfp）。

为了研究sks13的亚细胞定位，我们构建了两种亚细胞定位载体，即gfp序列分别融合在信号肽序列末端(gfp-sks13)及sks13羧基端(sks13-gfp),分别转入sks13-1突变体中。

2023年5月11日 · GFP的最大吸收峰为395nm（紫外），并有一个峰值为470nm的副峰（蓝光）；发射光谱最大峰值为509nm（绿光），并带有峰值为540nm的侧峰。 GFP的光谱特性与荧光素异硫氰酸盐（FITC）相似，所以GFP观察可用荧光显微镜滤光片组合。 GFP需在氧化状态下产生荧光，强还原剂能使GFP转变为非荧光形式。 GFP的优缺点及改进方法. GFP的优点. 易于检测，灵敏度高； 荧光稳定； 对细胞无毒害作用； 广谱通用性； 易于构建载体； 可进行活细胞 …

2016年4月20日 · In these lines, a T-DNA carrying GAL4-VP16 and modified UAS-driven GFP-gene (mGFP5ER) is randomly inserted into the Arabidopsis genome. A minimal CaMV 35S promoter is placed upstream of GAL4-VP16, and expression depends on insertion near an endogenous enhancer element.

2008年4月20日 · To test whether this growth effect resulted from a short-term perturbation of GA signalling, we monitored the effect of gai and GAI expression on the GA-responsive reporter RGA::GFP–RGA 3.