We fused the entire algD promoter, including the 5′ UTR, to gfp, essentially creating a transcriptional algD-gfp fusion. The algD-gfp construct was cloned into a mini-Tn 7 vector (pTn 7:: algD-gfp) suited for chromosomal insertion at a neutral genetic locus.

2007年11月15日 · We constructed mt-2(pPalgD-gfp) and mt-2(pPnptII-gfp), which harbor ectopic plasmid fusions of the promoters for algD and nptII, respectively. We examined the role of 1.5-MPa water potential reductions imposed by solute (NaCl, PEG 200, or …

2023年11月21日 · GFP-expressing Pst, Pst ΔalgU ΔmucAB (Pst algU mutant), and Pst ΔalgD ΔalgU ΔmucAB (Pst algU algD mutant) were examined to explore the roles of (1) alginate, a potential biofilm component; (2) Pst AlgU, thought to regulate alginate biosynthesis and some type III secretion system effector genes; and (3) intercellular salicylic acid (SA ...

2003年6月16日 · The first gene of this operon, algD, encodes GDP mannose dehydrogenase, an enzyme essential for alginate synthesis . The algD gene product catalyzes the first committed step in alginate synthesis and is not known to

2025年1月5日 · He et al. developed and evaluated novel amide derivatives as potent quorum-sensing inhibitors against P. aeruginosa PAO1 reporter strain, including lasB-gfp, rhlA-gfp, and pqsA-gfp (Fig. 24). All compounds inhibited lasB - gfp and rhlA-gfp expression by 1–82 % and 10–84 %, respectively, compared to the control DADS (36.08 % and 38.01 % ...

本研究发现流行克隆似乎对囊性纤维化（CF）或非囊性纤维化（non-CF）个体有内在偏好，并发现了与CF亲和性正相关和负相关的基因表达特征。 高CF亲和性的克隆更能在CF巨噬细胞内存活，部分是由严格反应调节因子 DksA1 的表达介导的，这表明某些流行克隆在感染CF患者方面的内在成功可能是由于增强的宿主先天免疫逃逸能力。 为了理解铜绿假单胞菌流行克隆通过多轮体内进化适应人类宿主的过程，研究检查了单个克隆的最近突变历史，识别了5641个基因中的224个 …

2018年9月25日 · We found that interactions between CdrA and the exopolysaccharide Psl fortify the matrix by preventing CdrA proteolysis. When both components—CdrA and Psl—are part of the matrix, robust aggregates form that are tightly packed and protease resistant. These findings provide insight into how biofilms persist in protease-rich host environments.

As shown in Fig. 2, the fluorescence of the arabinose-induced high-c-di-GMP-level strain (DGC1) was significantly higher than that of the uninduced DGC strain as well as the induced and uninduced...

During the course of chronic cystic fibrosis (CF) infections, Pseudomonas aeruginosa undergoes a conversion to a mucoid phenotype, which is characterized by overproduction of the exopolysaccharide alginate. Chronic P. aeruginosa infections involve surface-attached, highly antibiotic-resistant communities of microorganisms organized in biofilms.

2009年1月1日 · GDP-mannose dehydrogenase (GMD), encoded by the algD gene, is a key enzyme in the biosynthesis of alginate and catalyses the almost irreversible oxidation of GDP-mannose to GDP-mannuronic acid.