最大的基因载体——人类人工染色体(Human Artificial …
1997年，通过在人类HT1080细胞的端粒和基因组DNA中加入α-卫星(Alphoid)DNA，首次从头构建了HAC。 这就产生了一个全新的微染色体，其中包含了感兴趣的DNA，以及允许其结构和有 …
Alphoid sequences - Oxford Reference
2025年2月27日 · A complex family of repetitive DNA sequences found in the centromeric heterochromatin of human chromosomes. The alphoid family is composed of tandem arrays of …
Alphoid repetitive DNA in human chromosomes - PubMed
The thesis describes the first extensive DNA sequence analysis that demonstrated that the tandemly repeated alphoid DNA in the centromere of the human chromosomes consists of …
Human artificial chromosome: Chromatin assembly mechanisms …
2020年4月15日 · Both centromere chromatin and heterochromatin assemble on a centromeric DNA sequence, a highly repetitive sequence called alphoid DNA (α-satellite DNA) in humans. …
Alphoid DNA nucleotide sequences from two human cell lines …
1988年12月15日 · Alpha satellite DNA (alphoid DNA) is an abundant, highly reiterated sequence of constitutive heterochromatin present in the centromeric region of all human chromosomes …
CENP-B 盒阳性和阴性合成 alpha 卫星重复序列的组合改善了从头 …
通过将由高度重复的 171-bp α 卫星 (alphoid) DNA 组成的大 (>30 kb) 着丝粒序列引入 HT1080 细胞，可以从头形成人类人工染色体 (HAC)。 然而，只有一部分转化细胞成功地建立了 HAC。
Characterization of human centromeric regions of specific …
The alphoid DNA family is composed of tandemly repeated sequences whose organization is chromosome specific. Under stringent conditions of hybridization, subsets of these sequences …
Structure of the major block of alphoid satellite DNA on the ... - PubMed
1987年6月5日 · Alphoid DNA is a family of tandemly repeated simple sequences found mainly at the centromeres of the chromosomes of many primates. This paper describes the structure of …
An alphoid DNA sequence conserved in all human and great ape ...
Sequence analysis confirmed the alphoid nature of the whole pαH21 insert. Using vector-CENP-B box polymerase chain reaction (PCR) we isolated and cloned from a human chromosome 21 …
Human artificial chromosome: Chromatin assembly mechanisms and …
2020年4月15日 · Both centromere chromatin and heterochromatin assemble on a centromeric DNA sequence, a highly repetitive sequence called alphoid DNA (α-satellite DNA) in humans. …
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