2022年11月8日 · The Ciliary Adhesion (CA) complex forms in close association with the basal bodies of cilia during the early stages of ciliogenesis and is responsible for mediating complex interactions...

2021年6月29日 · Using Xenopus, we demonstrate that centriole number scales with apical area over a remarkable 40-fold change in size. We find that tensile forces that shape the apical area also trigger centriole amplification based on both cell stretching experiments and disruption of embryonic elongation.

Primary cilia are microtubule-based organelles that extend from the apical surface of most mammalian cells, forming when the basal body (derived from the mother centriole) docks at the apical cell membrane. They act as universal cellular “antennae” ...

2020年12月17日 · Primary cilia are microtubule-based organelles that extend from the apical surface of most mammalian cells, forming when the basal body (derived from the mother centriole) docks at the apical cell membrane. They act as universal cellular "antennae" in vertebrates that receive and integrate mechanica …

Human respiratory and oviductal cilia have specific apical structures characterized by a narrowed distal portion and a ciliary crown. These structures are conserved among vertebrates that have air respiration systems; however, the molecular components of these structures have not been defined, and their functions are unknown.

The apical structure of human respiratory cilia was studied using thin and thick sectioning techniques. The distal end of the mature cilium is narrowed and presents dense material and a crown-shaped glycocalyx. The crown is composed of three to seven bristles, having a mean length of 31 nm and showi …

2022年11月8日 · Whole-mount immunostaining of Xenopus embryos with an antibody against β-Tubulin gave strong staining of cilia, compromising our ability to image the intracellular MT network in mature MCCs. To overcome this, a deciliation strategy was employed to eliminate cilia-associated signals and facilitate visualization of intracellular MTs.

3 天之前 · Motile cilia perform crucial functions during embryonic development and in adult tissues. They are anchored by an apical actin network that forms microridge-like structures on the surface of multiciliated cells. Using Xenopus as a model system to investigate the mechanisms underlying the formation of these specialized actin structures, we observed stochastic bursts of intracellular calcium ...

2022年8月19日 · In this study, we developed a robust approach to engineer apical-out airway organoids (AOAOs) with cilia beating taking place at the organoid’s exterior surface, which effectively propelled the organoid to rotate when placed in 3D Matrigel support.

2007年6月1日 · Using a highly refined model of ciliogenesis developed in primary culture mouse tracheal epithelial cells (mTEC), we found that RhoA is required for the formation of a characteristic apical actin web in ciliated cells. The web is required for basal body docking and subsequent generation of ciliary axoneme.