2023年4月20日 · 从实验结果来看，在N2A和HEK 293T细胞中，与GFP与APP共表达相比， APOE2或APOE3与APP的共同表达明显增加了α-CTF的水平。 值得注意的是， APOE2与APP的共同表达显著减少了总Aβ40的产生。 不过，与对照组相比， APOE4和APP的共同表达未能改变α-CTF和Aβ40。 不难看出，APOE确实在调节APP的剪切，而且APOE2确实抑制了Aβ40的产生。 APOE对APP剪切的影响. 问题又来了，究竟是APOE的那个部分在调控APP的剪切呢？ 陈椰 …

2022年8月1日 · Confocal imaging and flow cytometry analyses showed that APOE3 treatment decreased the amount of SARS-CoV-2 pseudo-viruses in 293T-ACE2 cells in a dose-dependent manner (Fig. 1h–k), but had...

2023年6月21日 · Here, we report that human apolipoprotein E (ApoE), the most important genetic risk factor of sAD, interacts with γ-secretase and inhibits it with substrate specificity in cell-autonomous manners through its conserved C-terminal region (CT).

2018年11月29日 · Exogenous expression of ApoE in 293T cells reduces HIV-1 Env expression and viral infectivity. The results with MDMs suggested that ApoE, which is the newly-identified HIV-1-inducible cellular protein functions as the anti-HIV-1 factor by targeting Env.

Experiments using HEK 293T cells with trun-cation mutants of the ApoE isoforms showed that a-CTF levels were not significantly altered by co-expression of the NTs from ApoE2, -3, or -4 (Figures S2E and S2F). By contrast, a-CTF levels were elevated by more than 6-fold by the co-expression of com-mon ApoE CT (Figures S2G and S2H).

2022年3月28日 · We report that in aged human mesenchymal progenitor cells (MPCs), APOE accumulation is a driver for cellular senescence. By contrast, CRISPR–Cas9-mediated deletion of APOE endows human MPCs...

2024年5月28日 · Interestingly, ectopic apoE expression in HEK-293T cells increased the titers of tecVLPs (Supplementary Fig. 2g) and the effect of LDL-R blocking upon tecVLP transduction of Huh-7.5 target...

Western blot and qPCR analysis show that human ApoE is overexpressed in the AD-apoE3, AD-ApoE Kyoto, and AD-ApoE Sendai groups but is absent in the AD-eGFP and blank groups from publication...

Recent studies have shown that the lipidation and assembly state of apolipoprotein E (apoE) determine receptor recognition and amyloid-β peptide (Aβ) binding. We previously demonstrated that apoE secreted by HEK cells stably expressing apoE3 or ...

2018年11月29日 · Effects of ApoE3 mutants on Env expression and localization in 293T cells. (A) Schematic representation of ApoE3 protein structure and the deletion mutants (E3N, E3NΔ, E3C and E3CΔ). ApoE has three functional domains: the N-terminal signaling domain, the LDL receptor-binding domain, and the C-terminal lipid-binding domain.