2018年11月9日 · The AraC gene activates in pGLO from my understanding in the presence of arabinose. Arabinose is present as a metabolite from other metabolic in the e.coli cell. Is it that there's no termination marker, so transcription continues to the GFP gene? In addition, does AraC contain parts that are encoded 5'->3' and 5'->3'?

Explore Bio-Rad’s comprehensive pGLO and GFP teaching resources, including pGLO plasmid maps, hands-on lab activities, and free presentations, videos, and case studies. Learn how to use GFP to illuminate gene regulation and expression in your classroom.

The pGLO plasmid contains an origin or replication, a selectable marker, and the gene for Green Fluorescent Protein (GFP). The plasmid also contains a gene for the arabinose C protein, which is a protein that regulates expression from the arabinose BAD promoter (P BAD).

2011年11月11日 · Electroporation of the Tn-mutagenized pGLO plasmids into XL1-Blue cells yields reasonably large numbers of all three classes of mutants—β-lactamase, ara C, and GFP gene knockouts—and there is usually a surplus of mutant colonies to be picked.

There are glowing colonies surrounded by satellite colonies, because ARA turns on the araC gene which turns on the expression of the GFP gene. When this is expressed in the presence of UV light,...

2019年1月1日 · Binding of RNA polymerase to this promoter is controlled by a positive regulatory protein called AraC, which is encoded by a separate gene (araC) that has its own promoter site. The AraC regulatory protein forms dimers and can bind to …

2016年3月4日 · To do this, we need to find the protein that expresses the GFP gene, which is pGLO, so this is what we are really transferring to the E.coli. pGlO also codes for resistance to ampicillin so when the transformation will have two different phenotypes: glowing under UV light and the ability to reproduce when ampicillin is present.

The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably the green fluorescent protein (GFP) and the ampicillin resistance gene.

+pGLO, ampicillin, ara c, and +pGLO amp because it will take up all of the pGLO plasmid, which expresses the ampicillin resistant gene and makes the GFG gene fluoresce green

Three enzymes are produced; they do their job to break down arabinose, and eventually the arabinose runs out. The DNA code of the pGLO plasmid has been engineered to incorporate aspects of the arabinose operon. Both the promoter (PBAD) and the araC gene are present.