Anti-ATG8抗体(ab4753) - Abcam中文官网
Anti-Apg8 antibody, also known as Atg8, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. 1/4000 - 1/8000. Detects a band of approximately 14 kDa.
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Anti-ATG8 antibody (ab4753) - Abcam
Anti-Apg8 antibody, also known as Atg8, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
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Anti-APG8A/ATG8A抗体(ab98830) - Abcam中文官网
Suitable for: WB; Reacts with: Arabidopsis thaliana; Isotype: IgG; 你可能会感兴趣
自噬标志物—LC3检测要点 - 知乎 - 知乎专栏
微管相关蛋白1轻链3 (MAP1LC3)，是Atg8在哺乳动物中的同源物。 LC3/Atg8 被具有蛋白内切酶活性的 Atg4 在羧基端剪切，生成胞浆型 LC3-I。
【LC3跑WB方法】 - 丁香园论坛 - DXY.cn
LC3/Atg8 被具有蛋白内切酶活性的Atg4 在羧基端剪切，生成胞质LC3-I。 LC3-I 通过Atg7 和Atg3（分别对应E1 和E2 样酶）参与的泛素样反应，使磷脂酰乙醇胺（PtdEth，PE）偶联，生成脂质化形式的LC3，也称作LC3-II，它可以附着到自噬体（autophagosome）的膜上，是自噬体的 ...
ATG8 Rabbit pAb-多克隆抗体- ABclonal
Western blot analysis of lysates from Zea mays, using ATG8 Rabbit pAb (A22294) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
干货!《第4版自噬实验指南》中的Western Blot实验要点解读 - 知乎
Atg8家族蛋白 是自噬过程中的关键标记物，其包括LC3和 GABARAP 两个亚家族，它们在不同的自噬过程中有不同的功能。 LC3蛋白在自噬体的形成中起作用，而GABARAP亚家族成员则参与自噬体形成的后期阶段。 这些蛋白的脂化形式（如LC3-II和GABARAP-II）通常被用作自噬体的标记物，蛋白印迹是检测Atg8家族蛋白的关键方法。 在第4版“指南”中，详细的说明了Atg8家族蛋白的 Western Blot 实验方法和注意事项，总结如下： 1、新鲜样品应尽快煮沸并跑胶，不可反复进行 …
模式小物种抗体 -Atg8 Antibody - Abmart
Atg8 Antibody detects endogenous levels of total Atg8. Transmembrane. PBS, pH 7.4, 40% glycerol, and 0.01% sodium azide. Store at -20 °C. Stable for 12 months from date of receipt.Store at -20°C long term.
ATG8 Polyclonal Antibody (PA1-26648) - 赛默飞世尔科技公司
ATG8 Antibody (PA1-26648) in WB Western blot of APG8 fusion protein. ATG8 Polyclonal Antibody (Product # PA1-26648) generated by immunization with recombinant yeast APG8 was tested by western blot with other anti-UBL antibodies against E.coli lysates expressing the APG8-GFP fusion protein.
Anti-ATG8 Antibodies | Invitrogen - 赛默飞世尔科技公司
Antibodies that detect ATG8 can be used in several scientific applications, including ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. These antibodies target ATG8 in Yeast samples. Our ATG8 polyclonal antibodies are developed in Rabbit. Find the ATG8 antibody that fits your needs. Choose from 1 of 2 ATG8 antibodies,...
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