GFP-Atg8 protease protection as a tool to monitor …
Here, we describe an alternative method for examining autophagosome completion using GFP-Atg8 as a marker for protease protection. Perhaps the most complex step of macroautophagy …
四川大学卢克锋/许文明团队揭示生殖组织特异性自噬重要生理功能 …
为了验证Vac8在自噬中的重要作用，研究者分别在WT、Atg1Δ和Vac8Δ酵母细胞中过表达GFP-Atg8融合蛋白，通过GFP切割实验，发现Vac8缺失与Atg1缺失所导致的结果相似，均明显阻 …
For the last time, it is GFP-Atg8, not Atg8-GFP (and the same ... - PubMed
For the last time, it is GFP-Atg8, not Atg8-GFP (and the same goes for LC3)
For the last time, it is GFP-Atg8, not Atg8-GFP (and the same …
With a C-terminal fusion, GFP is cleaved from Atg8 without shifting to autophagy-inducing conditions, and even in an autophagy-defective mutant; however, the Atg8-GFP construct can …
自噬研究指南|干货篇：自噬检测方法详解_荧光_蛋白_实验
2023年8月25日 · 利用绿色荧光蛋白（GFP）在酸性环境中淬灭的特性，研究人员开发出了GFP-LC3工具检测自噬，可使用流式细胞仪定量检测，但使用荧光显微镜检测效果不够理想。
Autophagy formation, microtubule disorientation, and alteration of ATG8 …
2024年3月18日 · In selective autophagy, ATG8 plays a role in component binding, serving as a docking platform to adapters and receptors that harvest the cargo for further degradation in …
ATG8 lipidation and ATG8-mediated autophagy in Arabidopsis …
2010年4月16日 · Two markers of particular importance for investigating the yeast and animal ATG system are the ATG8–PE conjugate, which provides a biochemical marker for autophagic …
我院高彩吉课题组与合作者在Nature Plants发表重要研究结果 - 科 …
2023年4月20日，我校生物光子学研究院青年教师周俊博士与生命科学学院高彩吉课题组合作在自然子刊《Nature Plants》发表了题为“A non-canonical role of ATG8 in Golgi recovery from …
GFP-Atg8 蛋白酶保护作为监测自噬体生物发生的工具 …
2011年11月24日 · GFP-Atg8 protease protection as a tool to monitor autophagosome biogenesis. Perhaps the most complex step of macroautophagy is the formation of the double-membrane …
Role of Atg8 in the regulation of vacuolar membrane invagination
2019年10月15日 · In this study, we found that the expression of Atg8, an essential autophagy factor, is induced after chronic heat stress. In addition, without Atg8, vacuolar invaginations …