2025年3月11日 · Azido-dT Phosphoramidite is an excellent building block for introducing an azide group into an oligo via dT. Oligonucleotides bearing a single nucleosidic azide group can be readily modified with 2-5 equivalents of a tag-alkyne (e.g., a fluorescent dye alkyne).

2021年11月11日 · We have synthesized various 2′- and 3′-azido modified nucleosides and immobilized them on controlled pore glass (CPG) to be used as starting material for the synthesis of oligonucleotides (ONs) with 3′-terminal azide (attached to C2′ or C3′).

2019年9月23日 · Here, we introduce junctured-DNA tweezers as a generic platform that enables real-time observation, at the single-molecule level, of biomolecular interactions. This tool corresponds to a...

2022年2月21日 · We review here the available methods for the synthesis of various nucleosides decorated with azido groups at the sugar residue or nucleobase, their incorporation into oligonucleotides and cellular RNAs, and their application in azide-alkyne cycloadditions for labelling and functionalization.

Azide and phosphoramidite functions were found to be compatible within one molecule and stable for months in solution kept frozen at −20 °C. An azide-carrying phosphoramidite was used for direct introduction of multiple azide modifications into synthetic oligonucleotides.

Use azide modified oligos with Alkyne or DBCO Cyclooctyne- or BCN modifications for ease of copper catalyzed or copper-free click reagents. These are simple to use and has excellent click performance in 17 hours or less at room temperature.

IDT's Azide modification uses an NHS Ester functional group to attach an azide moiety at the 5', 3' or any internal position in an oligo. This azide moiety may subsequently be used to attach alkyne modified groups through the click reaction. The internal version of this modification is attached to the oligo through a dT base.

Azido-dT Phosphoramidite is an excellent building block for introducing an azide group into an oligo via dT. Oligonucleotides bearing a single nucleosidic azide group can be readily modified with 2-5 equivalents of a tag-alkyne (e.g., a fluorescent dye alkyne).

2020年5月18日 · Azido-labelled DCs were detected in lymph nodes for weeks, and could covalently capture dibenzocyclooctyne (DBCO)-bearing antigens and adjuvants via efficient Click chemistry for improved antigen...

We have synthesized various 2’-and 3’-azido modified nucleosides and immobilized them on controlled pore glass (CPG) to be used as starting material for the synthesis of oligonucleotides (ONs) with 3’-terminal azide (attached to C2’ or C3’).