Immune responses involving CD8⁺ T-cells are severely deficient, providing a model to assess the role of CD8⁺ cells and class I MHC in various experimental systems.

Fig1 B2m基因敲除纯合子小鼠CD8 + 细胞毒性T细胞很少，CD4 + T细胞代偿性增加。 CD8 + T细胞相关免疫应答严重缺陷，可用于评估CD8 + 细胞和I类MHC的作用。 相关品系

2022年3月1日 · Here, we generated a B2M homozygous knockout somatic cell nuclear transfer-induced embryonic stem cell (SCNT-ESC) line using CRISPR/Cas9-mediated gene targeting. …

2021年1月29日 · After knocking-out surface expression of HLA class I molecules by targeting the B2M gene via CRISPR/Cas9, we also co-expressed a single-chain HLA-E molecule, thereby …

2021年7月24日 · B2M KO: ~95%. TRAC KO: ~80%. CAR insertion into TRAC by AAV6 HDR: 60.6%. HLA-E insertion into B2M by AAV6 HDR: 73.4%. High efficiency Cas12a chRDNA …

2021年1月28日 · After knocking-out surface expression of HLA class I molecules by targeting the B2M gene via CRISPR/Cas9, we also co-expressed a single-chain HLA-E molecule, thereby …

NKG B2m KO mice are constructed by knocking out the B2m gene on the background of NKG mice. Compared with NKG mice, NKG B2m KO mice have a significantly longer survival …

2019年4月4日 · The HLA pseudo-homozygous iPSC approach would retain the surface expression of all HLA genes, offering an advantage over B2M KO cells engineered to express …

Highly efficient gene editing in primary myeloid cells is made possible by optimized non-viral delivery of CRISPR-Cas9. This enables single and multiplexed gene knockout in monocytes, …

2021年10月1日 · Here, we generated a homozygous B2M knockout induced pluripotent stem cell (iPSC) line, lacking the expression of HLA class I (HLA-I) molecules, using a CRISPR/Cas9 …