BL21 Competent E. coli is a widely used non-T7 expression E. coli strain and is suitable for transformation and protein expression. This strain does not express the T7 RNA Polymerase. Ideal for P lac, P tac, P trc ParaBAD expression vectors; Protease deficient

Thermo Scientific BL21(DE3) Competent Cells are suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.

BL21 (DE3) Competent E. coli is a widely used T7 expression E. coli strain. Ideal for routine T7 expression; Protease deficient B strain; No dry ice surcharge on competent cell shipments; Outgrowth medium included; Free of animal products

BL21 (DE3)菌株用于高效表达克隆于 含有噬菌体T7启动子的表达载体 (如pET系列)的基因。 λ噬菌体DE3区含有T7噬菌体RNA聚合酶，该基因整合于BL21的染色体上，所以称为BL21 (DE3)。 可同时表达T7 RNA聚合酶和大肠杆菌RNA聚合酶，用于pET系列，pGEX，pMAL等质粒的蛋白表达。 操作方法： 1.快速转化操作方法（10min） (1). 提前10分钟将用到的筛选培养基平板拿到37℃ …

2016年1月11日 · FAQ: What is the difference between BL21 and BL21(DE3) competent E.coli cells? Both strains are B strains and thus both are deficient in Lon protease (cytoplasm) and OmpT protease (outer membrane). Accordingly, B strains are generally preferred for recombinant protein expression.

Escherichia coli strain BL21 is one of the widely used bacterial hosts for high-level recombinant protein production and for other applications. Here, we present the complete genome sequence of a commercial version of the Escherichia coli BL21 strain.

Thermo ScientificTM BL21(DE3) Competent Cells are suitable for the expression of nontoxic heterologous genes. The strain contains the DE3 lysogen that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.

One Shot BL21(DE3) Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET. This strain had been engineered to carry the λDE3 lysogen that contains T7 RNA polymerase …

BL21-AITM One Shot® Competent E. coli is an arabi-nose-inducible strain designed to give you the maxi-mum protein expression with the tightest regulation available from a T7 expression system. It’s the only strain that gives you both tight regulation and high yields, making it great for high-level expression of.

2024年10月28日 · The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase.