Jun 7, 2023 · We developed a platform technology by genetically engineering the bovine papillomavirus L1 major capsid protein to insert a polyglutamic acid/cysteine motif in place of …

Mar 14, 2017 · In this preliminary study, we aimed to integrate data about BPV-1 L1 gene cloning and expression from a bacterial system (E. coli). Afterward, we characterized the L1 …

Mar 14, 2017 · Moreover, the absence of papillomavirus replication in vitro makes the use of recombinant protein a promising candidate for vaccine formulations. Hence, we developed an …

Jul 6, 2018 · Bovine papillomavirus-1 (BPV-1), the aetiological agent of equine sarcoids, primarily causes warts in its natural host, the cattle, it can also cause locally aggressive and invasive …

Oct 10, 2009 · The L1 major capsid proteins of BPV1 and BPV2 were expressed in Sf-9 insect cells and both self-assembled into virus-like particles (VLPs). Using conformation-dependent …

Bovine papillomavirus VLPs are 50 nm particles composed of 360 molecules of L1 proteins that form capsomeres of 5 L1 proteins and capsids of 72 capsomeres. The L1 protein was …

Mar 27, 2023 · Here, we describe the production of BPV1 L1 as VLPs in a baculovirus expression vector system (BEVS) and the purification of these particles by Lentil Lectin-Sepharose 4B as …

L2 possesses a second independent L1 interaction domain located between residues 129 and 246. This interaction was detected in vivo, but not in vitro. Both L1 interaction domains are …

Sep 1, 1996 · The papillomavirus major capsid protein L1 can assemble into capsids in vitro. To identify areas within the bovine papillomavirus type-1 L1 (BPV L1) protein that are important …

Hypothesis: BPV-1 L1 VLPs may constitute a safe and highly immunogenic vaccine candidate for protection of horses against BPV-1/-2-induced disease. Methods: Three groups of 4 horses …