2019年9月15日 · Treatment with bpV increased extracellular signal-related kinase (Erk) activity after scratch injury in vitro, and rapamycin reduced influence by bpV on Erk phosphorylation. After a cervical hemicontusive SCI, Akt phosphorylation decreased in total tissue via Western blot analysis ( p < 0.01) as well as in penumbral ventral horn motor neurons ...

Schematic diagram for proposed mechanisms of injury and bisperoxovanadium (bpV)-induced signaling changes in vivo and in vitro.Based on our findings, we believe bpV-mediated neuroprotection in spinal cord injury and an in vitro model of spinal neuron injury primarily through PI3K/Akt/mTOR signaling. The activation of extracellular signal-related kinase (Erk) also appears to play a role ...

2012年1月10日 · To determine whether SCI or bpV(pic) administration influenced PTEN's antagonism of PI3K and downstream mTOR activity, phosphorylated Akt (p-Akt) and p-S6 levels were assessed. 1 day following SCI, p-Akt significantly decreased in tissue including the injury epicenter (p<0.01), while p-S6 expression significantly increased (p<0.05). bpV-treated ...

2011年1月28日 · bpv (pic), a potent inhibitor for PTEN, significantly decreased infarct volume and reduced the number of TUNEL-positive cells. bpv (pic) prevented the injury-induced decrease in phosphorylation of mTOR, and the subsequent decrease in S6. Similarly, down-regulation of PTEN expression by RNAi also reduced the number of TUNEL-positive cells, and increased phospho-mTOR. PTEN deletion prevents ...

Sun et al. indicated that more follicles developed into secondary follicles when mTOR and PI3K stimulators (200 μM PA + 50 μM PP + 100 μM bpV + 250 μg/ml 740Y-P) were added in the culture medium of ovarian tissues, indicating a synergistic effect of mTOR and PI3K stimulators on human follicular development .

2016年1月1日 · The mammalian target of rapamycin (mTOR) and its protein complexes, complex 1 (mTORC1) and complex 2 (mTORC2) offer exciting and unique avenues of intervention in these diseases through the oversight of programmed cell death pathways of apoptosis, autophagy, necroptosis, and cellular metabolism. ... The use of bisperoxovanadium (bpV), ...

2024年8月28日 · Methods: The activity of mTOR and autophagy was evaluated in kidney samples from IgAN patients and in an IgAN mouse model induced by oral bovine serum albumin and carbon tetrachloride (CCl4) injection. mTOR inhibitors (rapamycin) and activators [bpV(phen)] were administered to the IgAN mouse model to observe the effects of mTOR on autophagy and ...

Treatment with bpV(pic) spares MNs in both in vivo and in vitro models of ALS. A The treatment schedule and experimental design for in vivo therapy of mSOD1 G93A ALS mice. B and D Ventral horn motor neurons were significantly less than wild-type (WT) mice at 90 days of age (p < 0.001).A late pre-symptomatic daily treatment schedule between days 70 and 90 of age enhanced the number of NeuN ...

2019年9月10日 · The protein kinase mTOR phosphorylates multiple downstream proteins of the PI3K/Akt pathway . In this experiment, p70S6K was selected as a biomarker of mTOR activity . The present study used pharmacological methods. The specific inhibitors of PTEN, Akt and mTOR, bpV(pic), LY294002 and ridaforolimus, were used to treat cells (10,42,43). The ...

2019年9月9日 · To validate the activity of bpV on PI3K/Akt/mTOR signaling, experiments were carried out using a PI3K inhibitor, LY294002, and an mTOR inhibitor, rapamycin, on injured spinal neurons. When neurons were treated with bpV and LY294002, the bpV-mediated increase in p-Akt at 30 min was significantly inhibited (86% decrease, Fig. 3A , p < 0.001).