2013年4月1日 · Zinc-finger recombinases (ZFRs) represent a potentially powerful class of tools for targeted genetic engineering. These chimeric enzymes are composed of an activated catalytic domain derived from the resolvase/invertase family of serine recombinases and a custom-designed zinc-finger DNA-binding doma …

2021年9月9日 · However, if their 7.6 second 0-62mph time doesn’t excite you, there are a handful of simple BRZ performance mods that can be bolted-on to give these small engines a little more oomph. With a few upgrades including tuning, a cold air intake, headers, exhaust, and E85 fueling, we can add about 50whp. All-in this will cost you a few thousand bucks.

Artificial zinc finger proteins (ZFPs) consist of Cys 2 -His 2 -type modules composed of ∼30 amino acids with a ββα structure that coordinates a zinc ion. ZFPs that recognize specific DNA target sequences can substitute for the binding domains of enzymes that act on DNA to create designer enzymes with programmable sequence specificity.

Zinc-finger recombinases (ZFRs) are chimaeric proteins comprising a serine recombinase catalytic domain linked to a zinc-finger DNA binding domain. ZFRs can be tailored to promote site-specific recombination at diverse 'Z-sites', which each comprise a central core sequence flanked by zinc-finger dom …

2013年2月6日 · We have shown that ZFRs can be assembled to recombine user-defined DNA targets, and that designed ZFRs integrate DNA into endogenous genomic loci. This work illustrates the potential of ZFRs for a wide range of applications, including genome engineering, synthetic biology and gene therapy.

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2019年11月28日 · 86/brz/frs 的改装潜力到底有多大？ 不得不说这车的底子真好，仅仅一些基础改装就有很大的提升。 你们的车圈速究竟提升了多少 显示全部

2017年8月17日 · Here, we present a straightforward set of protocols for the expression and purification of ZFRs, an assay system for catalytic proficiency in vitro and bandshift assays for detection of sequence-specific DNA interactions.

SUBARU BRZ的强劲动力，源自升级版2.4升D-4S ＊ 水平对置发动机（SUBARU BOXER），它放置在低重心的车身底部，提升操控性的同时，过弯时也能保持车身的平衡稳定。 *TOYOTA D -4S（Direct injection 4 s troke gasoline engine Superior Version）是丰田汽车株式会社的注册商标。

Zinc-finger recombinases (ZFRs) are chimaeric proteins comprising a serine recombinase catalytic domain linked to a zinc-finger DNA binding domain. ZFRs can be tailored to promote site-specific recombination at diverse ‘Z-sites’, which each comprise a central core sequence flanked by zinc-finger domain-binding motifs.