2022年10月1日 · Using bovine serum albumin (BSA), it was shown that staining did not depend on whether BSA was thermally unfolded in the presence of SYPRO Orange or stained after electrophoresis. On the contrary, SYPRO Orange dye stained protein bands in the presence of sodium dodecylsulfate (SDS) due to incorporation of the dye into SDS micelles that bound to ...

2019年12月4日 · The differential scanning fluorimetry (DSF) screening of 5.692 fragments in combination with benzenesulfonamide (BSA) against bovine carbonic anhydrase (bCA) delivered >100 hits that either caused, on their own, a significant thermal shift (ΔT m, °C) in the protein melting temperature or significantly influenced the thermal shift observed for ...

The differential scanning fluorimetry (DSF) screening of 5.692 fragments in combination with benzenesulfonamide (BSA) against bovine carbonic anhydrase (bCA) delivered >100 hits that either...

2019年12月4日 · The differential scanning fluorimetry (DSF) screening of 5.692 fragments in combination with benzenesulfonamide (BSA) against bovine carbonic anhydrase (bCA) delivered >100 hits that either caused, on their own, a significant thermal shift (ΔT m, °C) in the protein melting temperature or significantly influenced the thermal shift observed for ...

2024年3月18日 · We report a label-free differential scanning fluorimetry approach to determine PFAS–BSA binding over a broad temperature range. This method utilizes the tryptophan residue within the protein...

2021年1月15日 · Differential scanning fluorimetry (DSF) and calorimetry (DSC), as well as circular dichroism (CD), yielded insights on the stabilization and secondary structure of BSA upon incubation with the ILs.

2020年12月1日 · The differential scanning fluorimetry (DSF) screening of 5.692 fragments in combination with benzenesulfonamide (BSA) against bovine carbonic anhydrase (bCA) delivered >100 hits that either...

2022年10月1日 · Ferguson plot was used to characterize the multiple intermediate species of bovine serum albumin (BSA) upon thermal unfolding. Differential scanning calorimetry showed an irreversible melting of BSA in Tris-HCl and phosphate buffers with a …

2019年8月5日 · The measured melting temperature of bovine serum albumin (BSA) at a concentration of 1 mg/mL in phosphate buffer was 63 °C, which was close to the value of 64 °C obtained by DSC. The unfolding of BSA was confirmed using orthogonal techniques of second derivative ultraviolet-visible (UV-vis) spectroscopy and dynamic light scattering (DLS).

2019年7月25日 · 使用正交二阶紫外可见光谱（UV-vis）和动态光散射（DLS）的正交技术确认了BSA的展开。 该方法还能够揭示蛋白质的微环境变化，包括制剂效应。 还使用此方法测试了其他多个结构域的蛋白质，包括溶菌酶和IgG，并显示了与DSC相当的解链温度。