2020年10月6日 · a Representative western blot band showed the protein levels of Arg1 and iNOS, respectively representing M2- and M1-related gene in BV2 cells under LPS or IL-4 stimulation for 24 h in the...

2023年7月1日 · TIPE2 switches microglial polarization from M1 to M2 in BV2 cells treated with LPS. BV2 cells were incubated with or without LPS (1 μg/mL) for 24 h. Cells were stained with anti-CD86 (red) or anti-Arg1 (red) antibodies, nuclei were stained with DAPI (blue), and the staining results were observed by confocal (A-D).

2012年9月4日 · TGFβ enhances IL4-induced microglia alternative activation by strongly increasing the expression of Arg1 and Ym1. This synergistic effect on Arg1 induction is almost completely blocked by the application of the MAPK inhibitor, PD98059.

2019年4月25日 · Our results indicate that MCMV significantly alters the immunometabolic phenotypic properties of BV-2 microglia cells through the manipulation of iNOS and Arg-1 expression patterns, along with an induction of a glycolytic shift in the infected cell cultures.

Moreover, LXA4 upregulates the expression of the M2-associated Hes5, as well as the expression of the M2 microglia marker Arg1 and the associated inflammatory factor IL-10. These effects are blocked by the administration of the γ-secretase inhibitor DAPT, a specific blocker of the Notch signaling pathway.

使用免疫荧光染色、Western blotting方法检测缺血小鼠大脑中小胶质细胞和低糖刺激的BV2细胞中iNOS、MCT1及精氨酸酶1(ARG1)的表达。 用RNA干扰及质粒转染技术分别调控未处理BV2细胞中的MCT1表达，检测iNOS及ARG1的变化。

2021年6月1日 · Facts proved that the knockdown of cGAS induced an increase in microglial M2 polarization, reflecting as the elevated proportion of CD206/Arg1-labeled BV2 microglia and the isolated microglial cells from MCAO mouse brain. Meanwhile, the contents of TNF-α and iNOS in cell culture medium of BV2 cells-transfected with si-cGAS or in brain ...

While IL-4 up-regulated the M2 microglia polarization marker Arg1, which promoted microglia polarization toward a M2 phenotype (Fig. 1B). Moreover, LPS enhanced the protein expression of iNOS and...

与对照组比较，lps组细胞inos、cd32蛋白和mrna的表达增高，差异有统计学意义(p<0.05)；2组细胞arg1、cd206蛋白和mrna的表达差异无统计学意义(p>0.05)。 与对照组比较，LPS组细胞Notch1和Hes1蛋白和mRNA的表达明显增加，差异有统计学意义( P <0.05)；2组细胞Hes5蛋白 …

2024年9月28日 · Our results demonstrated that TA profoundly activated Arg1 in both mouse retinal microglia and peritoneal macrophages, suggesting universal effects of TA on Arg1 induction. Notably, among the M2 markers (Arg1, IL10) investigated in BV2 cells, only Arg1 was significantly upregulated by TA treatment (Fig. 6 B).