2017年7月4日 · Bafilomycin A1 (BafA1) is a specific and reversible inhibitor of vacuolar H+-ATPase (V-ATPase) with IC50 values of 4-400 nmol/mg. Bafilomycin A1, a macrolide antibiotic, is also used as an autophagy inhibitor at the late stage.

2010年8月25日 · Bafilomycins are specific inhibitors of vacuolar-type H+-ATPase. (V-ATPase). The most commonly utilized bafilomycin is bafilomycin A1. This is a useful tool as it can prevent the re-acidification of synaptic vesicles once they have undergone exocytosis. The next evolution of DrugBank is here.

Bafilomycin A1 (BafA1), a macrolide antibiotic isolated from Streptomyces species, is a specific vacuolar H + ATPase (V-ATPase) inhibitor. Notably, due to its ability to specifically target V-ATPase and hence disrupt autophagic flux, BafA1 is frequently used to study autophagy and endosomal acidification.

Bafilomycin is a potent macrolide antibiotic that acts as a specific inhibitor of vATPases, demonstrating the ability to inhibit bone resorption both in vitro and in vivo. You might find these chapters and articles relevant to this topic.

2021年3月19日 · Pharmacological inhibition of vacuolar-type H + -ATPase (V-ATPase) by its specific inhibitor can abrogate tumor metastasis, prevent autophagy, and reduce cellular signaling responses. Bafilomycin...

Bafilomycin A1 is an inhibitor of vacuolar H +-ATPase that is frequently used at high concentration to block late-phase autophagy. Here, we show that bafilomycin A1 at a low concentration (1 nM) effectively and specifically inhibited and killed pediatric B-cell acute lymphoblastic leukemia cells.

Bafilomycin A 1-dependent inhibition of the endoplasmic reticulum (ER) calcium pump Ca-P60A/SERCA induces a defect in autophagosome-lysosome fusion. Together, bafilomycin A 1 prevents cargo degradation and functional autophagic flux by acting at 2 distinct steps of the autophagic process.

Bafilomycin A1 (BafA) is a direct inhibitor of vacuolar ATPase, and interferes with the fusion of autophagosomes with lysosomes (Zhou et al., 2012).

6 天之前 · Isolated from Streptomyces griseus, bafilomycin A1 is a macrolide antibiotic that reversibly inhibits late phase autophagy (1,2). By inhibiting H+-ATPase, bafilomycin A1 prevents re-acidification of synaptic vesicles following exocytosis and thereby blocks fusion of autophagosomes and lysosomes (2-5).

2017年10月2日 · Bafilomycin A 1 (Baf) is a V-ATPase inhibitor that inhibits autophagosome degradation by blocking the fusion of autophagosomes with lysosomes, and this allows us to monitor autophagosome synthesis. 36,37 Immunohistochemistry and western blot experiments showed that the numbers of LC3B puncta and the LC3B-II protein expression level were both ...