In molecular biology, β-glucuronidase is used as a reporter gene to monitor gene expression in mammalian and plant cells. Monitoring β-glucuronidase activity through the use of a GUS assay allows determination of the spatial and temporal expression of the gene in question. [21]

The GUS reporter system (GUS: β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology [1] and microbiology. [2] Several kinds of GUS reporter gene assay are available, depending on the substrate used. The term GUS staining refers to the most common of these, a histochemical technique.

GUS基因就是β-葡萄糖苷酸酶基因 (β-glucuronidase)。 该基因产物为β-葡萄糖苷酸酶，属水解酶类，相当稳定而不易降解。 有简易的测定方法，且可使用市售的底物 (substrate) 在原位 (in situ) 显现出酶的活性，以肉眼即可检测其产物，非常方便。

2018年11月6日 · For the study reported herein, we examined the activities of GUSs from gut bacteria that represent five major groups of gut microbiota, namely Rg GUS, Cp GUS, Bf GUS, Ec GUS, and Bifd...

2022年1月20日 · GUS（β-glucuronidase，β-D-葡萄糖苷酸酶）基因是目前常用的一种报告基因，其表达产物β-葡萄糖苷酸酶是一种水解酶，能催化许多β-葡萄糖苷酯类物质的水解，它可以将5-溴-4-氯-3-吲哚-β-葡萄糖苷酸酯（5-bromo-4-chloro-3-indolyl-glucronide，缩写为X-Gluc）分解为蓝 …

2017年7月5日 · Microbiome-encoded β-glucuronidase (GUS) enzymes play important roles in human health by metabolizing drugs in the gastrointestinal (GI) tract. The numbers, types, and diversity of these proteins in the human GI microbiome, however, remain undefined. We present an atlas of GUS enzymes comprehensive for the Human Microbiome Project GI database.

The beta-glucuronidase (GUS) gene is used extensively in plant biology studies; this analysis summarizes its advantages and limitations. With the advances in genomic sequencing and computational analyses (including bioinformatics), its application in the study of plant gene expression is now an inte …

The reporter gene was the uidA gene of Escherichia coli that encodes the enzyme β-glucuronidase (GUS). Since then, the uidA gene (commonly referred to as the gus gene) has become one of the most widely used reporter genes in plant molecular biology. (For a detailed description of the gus gene, see Chapter 1.)

Beta-glucuronidase（GUS） reporter system is widely used in modern molecular biology, and playing vital roles in analysis of regulation of gene expression in time and space. This paper analyzed and summarized detection, biochemical characteristics, application and advanta...

Microbial β-glucuronidase (GUS) enzymes cause drug-induced damage in the mammalian gut. We identify the 3,013 total and 279 unique microbial GUS proteins in the Human Microbiome Project database, and reveal their differential processing of distinct substrates.