BHQ™ Probes are traditional, linear, dual-labelled FRET probes, typically 20 to 30 bases in length, with a fluorophore and quencher covalently attached to the 5' and 3' ends, respectively.

BHQplus Probes are an advanced probe type with duplex-stabilising chemistry to allow for the design of shorter oligonucleotides with relatively high melting temperatures. BHQplus Probes are used to detect difficult targets, such as AT-rich regions or SNPs.

BHQplus™ Probes are compact, dual-labelled, hydrolysis probes for qPCR. This probe type features a 5' fluorescent dye and a 3' Black Hole Quencher™ (BHQ) with the unique BHQplus duplex stabilising chemistry to enhance specificity and mismatch discrimination.

In addition to better spectral overlap, BHQ™ probes have much higher signal-to-noise ratios when compared to the corresponding DABCYL or TAMRA probes (Figure 4).

We introduced the Black Hole Quencher™ (BHQ™) dyes in 2000 and since then they have become the quencher of choice for qPCR probes and for other fluorescence-quenched probe applications.

BHQplus probes are a new and advanced probe technology for qPCR that bring researchers many of the benefits of traditional MGB probes without their expense. As with MGB probes, BHQplus probes form highly stable duplexes with DNA targets allowing shorter probes to be used for hybridization-based assays.

The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye.

A BHQ® probe is a dual-labeled oligonucleotide covalently labeled with a fluorophore and a Black Hole Quencher® (BHQ) dye. Q. What are the main components of a dual-labeled Black Hole …

2025年3月27日 · BHQplus™ Probes from LGC Biosearch Technologies are short dual-labeled hydrolysis probes available for qPCR and SNP genotyping. This probe type features a 3' Black Hole Quencher ™ (BHQ) and...